ZF RNA Extraction

marc marc at carl39.freeserve.co.uk
Wed Apr 6 08:45:00 EST 2005


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Hi again all,

Could anyone advise me on a protocol for extracting RNA from zebrafish
embryos.

I am using the small-scale SIGMA TRIZOL reagent protocol and i am
getting very poor results.  I usually de-corionate then isolate the RNA in
batches of ~20 embryos (0-24 hrs) and batches of 10 for 48 (hrs).

The yields are very poor, and the quality (in terms of ibosomal RNA) is
also poor.

If anyone uses this method or another method of RNA extraction i would
like to hear from them.

Cheers

Mark

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