ZF RNA Extraction

[Melissa Haendel]

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Hi Marc,
The Invitrogen TRIZOL reagent has worked very well for me in the past.  I
would try using a much larger number of embryos and follow the guidelines
for volume and mass (weigh a hundred embryos, for example, and use the
appropriate volume).  Also, are you using an electric tissue homogenizer? 
This really improves the yield as well.

Melissa Haendel
ZFIN curation staff

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>
> Hi again all,
>
> Could anyone advise me on a protocol for extracting RNA from zebrafish
embryos.
>
> I am using the small-scale SIGMA TRIZOL reagent protocol and i am
getting very poor results.  I usually de-corionate then isolate the RNA
in batches of ~20 embryos (0-24 hrs) and batches of 10 for 48 (hrs).
>
> The yields are very poor, and the quality (in terms of ibosomal RNA) is
also poor.
>
> If anyone uses this method or another method of RNA extraction i would
like to hear from them.
>
> Cheers
>
> Mark
>
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>

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