Zebrafish heads in resin
Heidi Barnes
h.barnes at ucl.ac.uk
Thu Apr 14 04:43:00 EST 2005
--------------------------------------------------------------------------
I am a histology BMS at the Institute of Ophthalmology doing paraffin
processed autopsy human brain & ophthalmic biopsies. Out of the blue I
have been asked to process, cut & tinctorially stain formalin fixed adult
zebrafish. The work requires sectioning through the undecalcified head to
prevent shrinkage involved in decalcification. The idea is that the
length of a population of cells in the eye will be measured in the
stained sections. Antibodies not needing to be demonstrated. Orientating
the removed eyeballs correctly would be difficult so half heads would be
used. I contacted Taab & they recommended Technovit 9100. I ordered a
kit but now find it is very difficult to use - polymerise at -10 in the
absence of air. I need to know :-
A) is there a resin which would support fishbone that is easier to handle
than this methyl methacrylate?
B) If I have to use this product could I see someone using it & see what
equipment they use to polymerised at room temp without excluding air.
Thank you very much indeed for any help you can give.
More information about the Zbrafish
mailing list