[Zbrafish] Embryo_Discard

[Melissa Haendel]

Hello Francois,
You can either put your embryos on a bucket of ice, or give them an 
overdose of ms-222 (tricaine sulfate).  Either way is humane.  The 
ms-222 would be better for lower volume of waste if the embryos are 
contaminated with your test compounds.

Melissa Haendel

françois wrote:

>Hello,
>
>I am working on Zebrafish embryo as a model to screen toxic compounds.
>My studies end up after 48hpf, some of my fish embryos are still
>"alive" and I would like to find the painless way to kill them.
>
>Does anybody know sthg about it?
>
>regards,
>
>François BUSQUET
>Merck KGaA
>Toxikologie Institute, U9/2025a
>Frankfurter Str. 250
>D-64271 Darmstadt
>
>
>_______________________________________________
>Zbrafish mailing list
>Zbrafish at net.bio.net
>http://www.bio.net/biomail/listinfo/zbrafish
>  
>

-- 

Melissa Haendel, Ph.D.

 

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