[Zbrafish] Embryo_Discard

Melissa Haendel mhaendel at uoneuro.uoregon.edu
Thu Aug 25 14:01:45 EST 2005

Hello Francois,
You can either put your embryos on a bucket of ice, or give them an 
overdose of ms-222 (tricaine sulfate).  Either way is humane.  The 
ms-222 would be better for lower volume of waste if the embryos are 
contaminated with your test compounds.

Melissa Haendel

françois wrote:

>I am working on Zebrafish embryo as a model to screen toxic compounds.
>My studies end up after 48hpf, some of my fish embryos are still
>"alive" and I would like to find the painless way to kill them.
>Does anybody know sthg about it?
>François BUSQUET
>Merck KGaA
>Toxikologie Institute, U9/2025a
>Frankfurter Str. 250
>D-64271 Darmstadt
>Zbrafish mailing list
>Zbrafish at net.bio.net


Melissa Haendel, Ph.D.


ZFIN Scientific Curator

Zebrafish Information Network

5291 University of Oregon

Eugene, OR 97403-5291

Phone: (541) 346-5108

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