Pronase enzyme

marc marc at
Wed Feb 16 03:07:00 EST 2005


Could anyone advise us on the following: we are going to buy a pronase
enzyme for use in de-corionation of the zf embryos.

I have sourced some from Fluka (SIGMA).  Do you know of any problems
associated with this method of de-corionation, i.e. is there problems with
batch-to-batch variability or enzyme purity considerations??

Mark Carlile

Institute for Cell & Molecular Biosciences
Medical School
University of Newcastle upon Tyne


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