in situ pigment problem

alanmayer at mac.com alanmayer at mac.com
Thu Mar 24 12:48:00 EST 2005


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Periodically (and always at the worst times) PTU'ed embryos will
develop pigment (melanin) at the end of an in situ, upon exposure to the
alkaline Tris buffer (NTMT).  Has anybody experienced this and know how to
avoid it?

Thanks!

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