[Zbrafish] tamoxifen-induced gene expression in zfish?

[Gilbert Weidinger]

Hi all,

I wonder whether anybody has tried to use tamoxifen to induce expression of genes fused to the 
estrogen receptor? In particular, is anybody out there who has tried the Cre-ER T2 construct, which 
is being used in mice to induce Cre recombination in response to tamoxifen-treatment?

I would like to get info on:

1) toxicity of tamoxifen in zebrafish (embryos and adults)
2) effectiveness (does it actually induce expression at non-lethal doses)
3) tightness of regulation (are ER-fused constructs expressed without tamoxifen)

any help is greatly appreciated!
gilbert
-----------------------
Gilbert Weidinger
Moon lab
HHMI/Dept. Pharmacology
University of Washington
Campus Box 357370
Health Science Bldg, Room K-522
1959 NE Pacific St
Seattle, WA 98195
USA

Tel. 001 206 685 2891
Fax. 001 206 543 0858
email. gweidin at u.washington.edu