[Zbrafish] In situs on larval stage fish

[Adrian Grimes]

Hi!
Does anyone have a protocol for removing pigment on later stage larvae for 
in situ hybridizations? Can I use hydrogen peroxide and, if so, at what 
point in a standard in-situ protocol should I attempt this? I don't want to 
use PTU as it may complicate the toxicology study I am doing.
Thanks,
Adrian Grimes

"Nothing is more common than the erroneous belief that one is displaying
judgment or taste by showing an unwillingness to be pleased"  Samuel Johnson
(1709 - 1784)
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Adrian Grimes
PhD Candidate
c/o The Kirby Lab
Neonatal Perinatal Research Institute
Box 3179 Bell Building
Duke University Medical Center
Durham
NC 27710

Tel: (919) 668-2311
Fax: (919) 668-1599