[Zbrafish] Large bands in in-situ probes

catsglynn catsglynn at gmail.com
Tue Aug 29 08:10:28 EST 2006


Hi,
I'm trying to make anitsense and sense probes (450bp) for in-situ
hybridizations but I'm frequently getting a larger band of about 3kb in
size. I have used SacI and SacII to digest the insert from PGEMTeasy,
and it has digested completely. However, I keep getting a larger band,
either in the sense or antisense probe. Has anyone encountered this
before? Can anyone help me please?
Thank you,
Catherine.

Catherine Glynn, M.Sc.,
Lucy Byrnes' Lab,
Department of Biochemistry,
National University of Ireland, Galway,
Ireland.



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