[Zbrafish] ENU safety precautions

[Christian Broesamle]

Hi everybody,

We are getting ready for a round of ENU 
mutagenesis and have to think about the 
approriate safety precautions. One concern is the 
location where the ENU treatment will take place. 
Most labs seem to use a fume hood for the 
mutagenesis setup. The people I talked to gave 
varying reasons from "we do all our nasty stuff 
there" to concerns about vapors and aerosols but 
with little concrete background.

I was wondering whether anyone has a more 
educated opinion, for instance on vapor pressure 
of ENU (I couldn't find anything in the MSDS of 
SIgma) or aerosol formation from a fishtank.

Has anyone done mutagenesis outside a fume hood? 
If so, what was the setup like?

Thanks for your input,

Christian
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Christian Brösamle
Department of Biochemistry
Ludwig-Maximilians-Universität München
Schillerstrasse 44
80336 München
Germany
tel: +49 89 2180 75-451
fax:  +49 89 2180 75-415
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