[Zbrafish] ENU safety precautions

[Christian Broesamle]

Mike,

Don't get me wrong. I am not looking for 
shortcuts.  I have done ENU mutagenesis before ( 
in a different place) and of course will do it 
with the full program of personal and 
environmental protection (as you describe it). I 
was just wondering whether there are any hard 
data that would help in designing the safest 
protocols. I could for instance imagine that a 
quiet, dedicated, spacious room is superior to a 
small, cramped hood in a lab that is usually 
shared with others.

You wrote the major danger is inhalation. Is this 
only in regard to the powder or also once the ENU 
is dissolved?

Christian


>Christian,
>
>ENU is not to be trifled with. We use the fume hood as a containment.
>Every surface in the hood is covered with lab 
>paper and discarded between treatments to 
>minimize potential contamination for the next 
>user of the fume hood.
>The major danger lies in inhaling the compound 
>followed by skin contact. We dissolve the powder 
>in the ISOPAC® provided where as others choose 
>to dump the powder into a beaker to dissolve.
>Aerosolizing is minimized by syringing the 
>solution into the buffered egg water. Guess its 
>comforting to know the venting action of the 
>hood will assist in the event of a mistake.
>We double glove and wear Tyvec coveralls... call it useful paranoia.
>
>Bill Trevarrow at U of Oregon is a good contact for alternative methods:
>Email:
><mailto:trevarro At uoneuro.uoregon.edu>trevarro At uoneuro.uoregon.edu
>
>
>Good luck,
>
>Mike
>
>
>   Michael C. Kacergis
>   U Mass Medical
>   Program in Gene Function and Expression (PGFE)
>   364 Plantation St.
>   LRB 670Q
>   Worcester, MA 01605
>
>   email: <mailto:Michael.Kacergis At Umassmed.edu>Michael.Kacergis At Umassmed.edu
>   (508) 856-2605 LAB
>   (508) 856-5460 FAX


--
***********************************
Christian Brösamle
Department of Biochemistry
Ludwig-Maximilians-Universität München
Schillerstrasse 44
80336 München
Germany
tel: +49 89 2180 75-451
fax:  +49 89 2180 75-415
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