[Zbrafish] zn-5 and zn-8 antibodies

[Chi-Bin Chien]

Hi Jill,

My understanding has always been that zn-5 and zn-8 came out of 
different wells in the same hybridoma screen (done by Bill 
Trevarrow), and thus likely represent two different isolates of the 
same clone (which would thus recognize the same epitope).  Dave 
Fashena worked on DM-GRASP and these antibodies for his PhD thesis. I 
believe that they work on Western, I forget if you have to use 
nonreducing conditions.

I hope that Dave or Bill can correct or amplify.

Chi-Bin Chien

At 8:54 AM -0800 2/13/06, Jill Sakai wrote:
>Hi all,
>I have frequently seen zn-5 and zn-8 antibodies used and/or referenced
>interchangeably, and both bind DM-GRASP.  Does anyone know if they were
>generated separately and thus are likely to bind different epitopes of
>the DM-GRASP protein?
>
>Also, does anyone have any experience using either zn-5 or zn-8 on
>Western blots?
>
>Thanks very much,
>Jill Sakai
>jasakai at wisc.edu
>
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