[Zbrafish] zn-5 and zn-8 antibodies

Bill Trevarrow trevarro at uoneuro.uoregon.edu
Mon Feb 13 13:53:01 EST 2006


Hi Jill,

Chi-Bin's summary is correct. Both zn-5 and zn-8 
can from the same mouse, but were found in 
different plates.


>Hi Jill,
>
>My understanding has always been that zn-5 and 
>zn-8 came out of different wells in the same 
>hybridoma screen (done by Bill Trevarrow), and 
>thus likely represent two different isolates of 
>the same clone (which would thus recognize the 
>same epitope).  Dave Fashena worked on DM-GRASP 
>and these antibodies for his PhD thesis. I 
>believe that they work on Western, I forget if 
>you have to use nonreducing conditions.
>
>I hope that Dave or Bill can correct or amplify.
>
>Chi-Bin Chien
>
>At 8:54 AM -0800 2/13/06, Jill Sakai wrote:
>>Hi all,
>>I have frequently seen zn-5 and zn-8 antibodies used and/or referenced
>>interchangeably, and both bind DM-GRASP.  Does anyone know if they were
>>generated separately and thus are likely to bind different epitopes of
>>the DM-GRASP protein?
>>
>>Also, does anyone have any experience using either zn-5 or zn-8 on
>>Western blots?
>>
>>Thanks very much,
>>Jill Sakai
>>jasakai at wisc.edu
>>
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-- 

Bill

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Bill Trevarrow
Zebrafish Facility Director
Institute of Neuroscience
University of Oregon 1254
Eugene, OR 97403-1254
Off. Tel: (541) 346-4598
Fac. Tel: (541) 346-4512
Fac. Website: http://fish.uoregon.edu/zf/
Fax: (541) 346-4548
e-mail: trevarro at uoneuro.uoregon.edu



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