Hi Jill, I needed to use non-reducing conditions to get my zn-5 westerns to work. You can find more details in J. Comp. Neurol. 406(3):415-424. Here's the publication record in ZFIN: http://zfin.org/cgi-bin/webdriver?MIval=aa-pubview2.apg&OID=ZDB-PUB-990507-16 have fun, dave_fashena