[Zbrafish] whole mount in situ hybridization problem

SmallPotato jxie at unity.ncsu.edu
Sun Jul 23 09:10:59 EST 2006

I have cloned my gene using RT-PCR. Now I want to see its expression
pattern using whole mount ISH. My postive control using the other probe
works. But my gene have trouble in getting signals.I have tried 1.1kb
and 2.0kb probe. None of them works.Do you have any suggestions?

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