[Zbrafish] Re: whole mount in situ hybridization problem

Cheuk-Chung Tony Ho wind.4ever at gmail.com
Wed Jul 26 04:52:23 EST 2006


For starters, what protocol did you followed? and what is the
hybridization and wash condition? I racken you use DIG-labelled probe,
mate?

Tony



SmallPotato wrote:
> Hi,
> I have cloned my gene using RT-PCR. Now I want to see its expression
> pattern using whole mount ISH. My postive control using the other probe
> works. But my gene have trouble in getting signals.I have tried 1.1kb
> and 2.0kb probe. None of them works.Do you have any suggestions?
> Thanks,



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