[Zbrafish] Chordal structure Malformation
(by busquet.francois At gmail.com)
Tue Nov 21 07:51:51 EST 2006
since one week, a third of my fish embryos incubated in fish medium at
26°C, developed chordal structure malformation like a ,,zigzag"
after 24hpf. I didn't know from which aquarium it comes from because
I collected all the eggs from the boxes in one big petri dishe.
Therefore, I first checked in which aquarium this could come from. I
collected once again fish eggs depending on the aquarium origin and I
incubated the fish eggs in fish medium at 26°C.
Today, I figured out that more than 80% fish embryos displayed again
chordal structure malformation for every aquarium. Moreover, I changed
all the population for one aquarium last weekend and replaced by
younger ones (around 8 months) but in the same water. It didn't make
any differences, the chordal malformation was also for 80% of fish eggs
for this aquarium.
We use a static system where fish medium is totally replaced every 15
days. The fish medium is based on deionized water + CaCl2 + MgSO4
(with Ca:Mg ions is 4:1) + NaHCO3 + KCl (with Na:K ions is 10:1). I
heard that chordal structure malformations could be linked to heavy
metals presence or from some infectious diseases or even vitamin
Any comment are very welcome!
PhD student/ Ecotoxicological Institute/ Merck KgaA/ Germany.
More information about the Zbrafish