[Zbrafish] Re: Help needed with Fish room practices

Tiago Filipe Baptista da Rosa Repolho via zbrafish%40net.bio.net (by trepolho from bio.ua.pt)
Mon Oct 9 05:13:05 EST 2006


Hello Becky,

There are some you posted that I can´t help you out once 
we don´t have a kind of "University animal Care group", 
altough I can give you some suggestions concerning other 
questions that you have posted:

>2) Are you required to wear disposable lab coats, booties, and/or face masks to enter your facility?

We are not required to wear any kind of personal 
protection equipment whereas the fish facility is 
concerned altough some precautions are made, i.e.:
Wearing a lab coat which is a standard lab request and 
above all, disposable gloves (not powdered) such as 
nitrile ones, first to protect our selves and second to 
avoid any kind of contamination getting in the water.
  
>3) What do you label your tanks with? Does anyone else use tape? Is this an issue?

When I worked at Oxford, there wasn´t any issue concerning 
the use of tape on tanks, at least from my point of view 
and the Home Office Inspector.
Also from the Veterinary Services that wasn´t an issue.
You could try to use PVC tape, which is a tape that I use 
that doesn´t leave any kind of residue on the tanks (At 
least on Marine Biotech as well as Aquarien Bau-Scharwz 
tanks). You can find them in general stores, differnet 
sizes and colours, so you can tag your tanks according to 
fish stage, numbers, strain, etc....
What I normally do is to tag the tanks in the frontal side 
and put the tag in a position that enables it not to enter 
in contact with the water and also enables people to 
identify the tank and fish in a rapid and correct way.

>4) How do you deal with rust in your facilities?

We do not have any kind of rust in our facilities whereas 
fish systems as well as other equipment that is in direct 
contact with water is concerned.
As long as there is any metal "things" within the fish 
facility, there is always the possibility of rust once you 
always have a high RH value in the rooms.
But as long as it doesn´t enter in contact with the water, 
that should not be a problem.

>5) How do people feel about using paint or other chemicals that leave fumes in the facility?

At our facility is not allowed to use or perform any kind 
of procedures that might produce an aerossol.
Special care should be taken once there are some 
"airborne" chemicals that can go into the water.
I think the rule should be: The Fish facility should only 
be for the rearing and culture of fish, nothing else.
If you want to perform any other experiments besides fish 
crossings, water testing, you should perform them in a 
separate room.

Hope I could help,

Tiago Repolho



Em Fri, 6 Oct 2006 11:05:29 -0500 (EST)
  zbrafish-request from oat.bio.indiana.edu escreveu:
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> 
>   1. (no subject) (Adelino Canario)
>   2. tdTomato use? (Chi-Bin Chien)
>   3. Zebrafish kidney (Claudia)
>   4. Staining of degenerating neurons. (Madhusmita 
>Priyadarshini)
>   5. Help needed with Fish room practices (Burdine, 
>Rebecca D)
>   6. Re: Help needed with Fish room practices (Thomas 
>Bartman)
> 
> 
> ----------------------------------------------------------------------
> 
> Message: 1
> Date: Fri, 28 Oct 2005 10:17:19 +0100
>From: Adelino Canario <acanario from ualg.pt>
> Subject: [Zbrafish] (no subject)
> To: bionet-organisms-zebrafish from moderators.isc.org
> Message-ID: <djsq1i$1nml$5 from cronus.fccn.pt>
> Content-Type: text/plain; charset=windows-1252; 
>format=flowed
> 
> 1) Post doc position “Functional genomics of Fish 
>environmental Adaptation”
> 
> 
> Commencing date: As soon as possible, after the closing 
>date
> Place of work: Centre of Marine Sciences, Univeristy of 
>Algarve, Campus 
> de Gambelas, 8005-139 Faro, Portugal.
> 
> Job description:  This project is developed within the 
>framework of the 
> Network of Excellence Marine Genomics Europe, which 
> implies 
> collaboration and coordination between several 
>laboratories aimed at 
> developing and applying cDNA and oligo microarrays. The 
>research 
> objectives are to identify genes and gene networks 
>involved in the 
> adaptation of fish to changing environmental conditions.
> 
> Qualifications and experience: Candidates must hold a Ph 
>D and have 
> experience in microarray analysis.
> 
>Further information can be obtained from prof. Adelino V. 
>M. Canário: 
> acanario from ualg.pt
> 
> Contractual conditions: Initial 2-year contract, in 
>accordance with 
> current fellowship legislation and for the 
>implementation of the project.
> 
> Closing date: 10 November 2005.
> 
> To apply for this position, candidates should submit a 
>detailed CV, with 
> concise description of research experience and the names 
>and addresses 
> of at least two referees quoting ref. CCMAR /BPD/0025/ 
>2005 
> to:ccmar from ualg.pt
> Centro de Ciências do Mar, Universidade do Algarve, 
>Campus de Gambelas
> 8005-139 Faro, Portugal
>Fax: +351 289 818 353      E-mail:  ccmar from ualg.pt
> 
> 
> 
> ------------------------------
> 
> Message: 2
> Date: Thu, 5 Oct 2006 10:57:10 -0600
>From: Chi-Bin Chien <chi-bin.chien from neuro.utah.edu>
> Subject: [Zbrafish] tdTomato use?
> To: zbrafish from magpie.bio.indiana.edu
> Message-ID: <p06230902c14ae8d322b0@[155.101.247.156]>
> Content-Type: text/plain; charset="us-ascii" ; 
>format="flowed"
> 
> Hi everyone,
> 
> I'm writing to ask if anyone else has experience with 
>expressing 
> tdTomato in zebrafish embryos. This is an mRFP variant 
>from the Tsien 
> lab (Shaner et al., 2004) that is supposed to be 
>extremely bright. We 
> have had the following odd result.
> 
> We cloned tdTomato into pCS2+, which allows either in 
>vitro 
> transcription of mRNA, or expression from DNA using the 
>sCMV 
> promoter. When we inject DNA at the 1-cell stage, we see 
>nice mosaic 
> expression of red fluorescence, as expected. However, 
>when we make 
> mRNA and inject it, we get no fluorescence whatsoever. 
>Other pCS2+ 
> fluorescent protein constructs work perfectly fine in 
>our hands when 
> we inject mRNA.
> 
> We would very much like to hear if others have had good 
>(or bad) 
> results with tdTomato. On paper it is a great 
>fluorophore, so we 
> would like to figure out what's happening.
> 
> Thanks,
> Chi-Bin Chien
> -- 
> < Dept. Neurobiology and Anatomy |       office: 
>1-801-585-1701 >
> < Univ. Utah Medical Center      |          lab: 
>1-801-585-1702 >
> < 20 North 1900 East, 401 MREB   |          fax: 
>1-801-581-4233 >
> < Salt Lake City, UT  84132      | 
>chi-bin.chien from neuro.utah.edu >
> < http://chien.neuro.utah.edu 
>                                  >
> 
> 
> 
> ------------------------------
> 
> Message: 3
> Date: 6 Oct 2006 05:13:58 -0700
>From: "Claudia" <claudia_hohn from hotmail.com>
> Subject: [Zbrafish] Zebrafish kidney
> To: bionet-organisms-zebrafish from moderators.isc.org
> Message-ID: 
><1160136838.880417.151820 from k70g2000cwa.googlegroups.com>
> Content-Type: text/plain; charset="iso-8859-1"
> 
> Hi,
> I am reading a lot of articles where kidney tissue was 
>isolated e.g.
> for flow-cytometry... I was wondering if somebody could 
>give me an
> insight on how to disect kidney in zebrafish. Whenever I 
>disect a fish
> I can not even see kidney tissue (only in tissue 
>sections under the
> microscope).
> Thank you,
> Claudia
> 
> 
> 
> ------------------------------
> 
> Message: 4
> Date: Fri, 6 Oct 2006 15:22:38 +0300
>From: "Madhusmita Priyadarshini"
> 	<madhusmita.priyadarshini from helsinki.fi>
> Subject: [Zbrafish] Staining of degenerating neurons.
> To: <Zbrafish from magpie.bio.indiana.edu>
> Message-ID: 
><005201c6e942$1cce6700$913cd680 from ltdk.ad.helsinki.fi>
> Content-Type: text/plain;	charset="iso-8859-1"
> 
> Hello everybody,
> 
> Does anybody use some staining procedure for 
>degenerating neurons or regenerating neurons?
> Has anybody the idea of the silver staining protocol for 
>the same?
> 
> Thank you.
> BR,
> Madhu
> 
> 
> 
> ------------------------------
> 
> Message: 5
> Date: Fri, 6 Oct 2006 10:46:02 -0400
>From: "Burdine, Rebecca D" <rburdine from Princeton.EDU>
> Subject: [Zbrafish] Help needed with Fish room practices
> To: <zbrafish from magpie.bio.indiana.edu>
> Message-ID:
> 	<9A199EA7817D8844955074BB433A74A503520B from MBCLUSTER.pu.win.princeton.edu>
> 	
> Content-Type: text/plain; charset="us-ascii"
> 
> Hi everyone,
> 
> 
> 
> Our fish facility at Princeton is under my supervision 
>and animal care
> has minimal input into our facility.  The only thing 
>they do is take the
> trash out that we place in the hallway.  However, each 
>time we come up
> for inspection by the University Committee on Animal 
>Care or larger
> inspections from the AAALAC, we begin to have some 
>disagreements with
> the departmental animal care staff.
> 
> 
> 
> I would like to hear how other fish facilities are run 
>with respect to
> animal care and proper AAALAC practices.  In addition, 
>if anyone has
> answers to the questions below, I would appreciate it.
> 
> 
> 
> 1)       Does a University Animal Care group run your 
>facility?  If not,
> how much input do they provide?
> 
> 2)       Are you required to wear disposable lab coats, 
>booties, and/or
> face masks to enter your facility?
> 
> 3)       What do you label your tanks with?  Does anyone 
>else use tape?
> Is this an issue?
> 
> 4)       How do you deal with rust in your facilities?  
> 
> 5)       How do people feel about using paint or other 
>chemicals that
> leave fumes in the facility?
> 
> 
> 
> 
> 
> Any input would be greatly appreciated! 
> 
> 
> 
> Becky
> 
> ---------------------------------------------------
> 
> Rebecca D. Burdine, Ph.D.
> 
> Assistant Professor
> 
> Dept. of Molecular Biology
> 
> Princeton University
> 
> Washington Road Mof 433
> 
> Princeton, NJ 08544 
> 
> 
> 
> Phone: (609) 258-7515
> 
>Fax: (609) 258-1343
> 
> Email: rburdine from princeton.edu
> 
> Admin Assistant: Cathy Falk (609) 258-1604
> 
> 
> 
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> ------------------------------
> 
> Message: 6
> Date: Fri, 06 Oct 2006 12:04:04 -0400
>From: Thomas Bartman <thomas.bartman from cchmc.org>
> Subject: Re: [Zbrafish] Help needed with Fish room 
>practices
> To: "Burdine, Rebecca D" <rburdine from Princeton.EDU>
> Cc: Zbrafish from magpie.bio.indiana.edu
> Message-ID: 
><6.0.0.22.2.20061006114329.01d27900 from mercury.chmcc.org>
> Content-Type: text/plain; charset="us-ascii"
> 
> Becky,
> 
> I have posted to this group before with similar issues 
>about 2-3 years ago 
> as I was getting a facility started here in Cincinnati. 
> You might be able 
> to look back and see people's answers to my original 
>query.  However, I 
> have had experiences since then, and can fill you in 
>(AAALAC is actually on 
> our campus today).  Answers to your questions (by 
>number).
> 
> 1) When I arrived here, I aimed for the model you have - 
>no involvement of 
> Veterinary Services whatsoever - because that is what I 
>had seen from where 
> I came.  But, Veterinary Services here expected total 
>involvement, like 
> they would with mice or any other animal.  So, we came 
>at it from very 
> different perspectives which led to a few years of 
>bitterness.  We have 
> reached a point where we both agreed that we had 
>different strengths and 
> weaknesses, and we would find a way to complement 
>instead of antagonize 
> each other.
> 
> Our facility is located in space assigned to Veterinary 
>Services, although 
> separate from the main animal facility.  My lab and I do 
>all but the 
> simplest maintenance - we do the feeding, fry rearing, 
>changes of in-line 
> filters, adjusting of water quality, etc.  They change 
>filter pads under 
> the racks, check each tack each day and leave a note of 
>their concerns 
> (sick looking fish, dirty tanks, inadequate water flow, 
>etc.) which we 
> correct, and they do all documentation for AAALAC, etc. 
>(keep logs of water 
> quality, maintenance tasks that we perform, etc.).  So, 
>they are pretty 
> hands-off, but do take care of all sorts of paperwork, 
>etc. that is 
> required for regulatory agencies.  I can imagine if/when 
>I get more 
> comfortable with their understanding of the animal and 
>the facility that I 
> may gradually transfer some more tasks to them, although 
>I am not ready to 
> do that yet.
> 
> I do pay a per-tank per diem for their oversight.  In 
>one sense, I think it 
> is silly, but on the other hand my lab/division does not 
>pay overhead on 
> the space so it still probably works out in my favor, 
>and the rate is 
> acceptably low.  And, they do make our job a little 
>easier when they point 
> out a sick/dead fish that we didn't notice during our 
>feeding.
> 
> 2) No.
> 
> 3) We use typical laboratory tape (various colors from 
>Fisher).  It was an 
> issue that Vet Services raised, because AAALAC has 
>criticized it at 
> times.  However, I felt there was no good alternative - 
>especially since we 
> wanted a quick way to identify the lab member 
>responsible for each tank.  I 
> spoke with Dr. Kathryn Bayne, director of accreditation 
>at AAALAC 
> (301-231-5353), and asked if laboratory tape could be 
>used in the fish 
> facility.  She was a pleasant woman and we had a very 
>nice 
> conversation.  She stated that there is no fixed rule 
>against using tape, 
> that no one has ever lost accreditation because of tape, 
>and that she has 
> seen taped used in other facilities.  She said a 
>worst-case scenario would 
> be that its use would be mentioned as a "Suggestion for 
>Improvement", which 
> is something that institutions are free to ignore with 
>no consequences (and 
> she doubted that it would even get listed at all).
> 
> She stated that their concern, which could theoretically 
>cause tape to be 
> listed as a "Suggestion for Improvement", is that 
>residue left behind when 
> tape is removed could collect dust and grime.  Given 
>that, she said that if 
> we documented a) that we were aware of this potential 
>concern, b) we had a 
> mechanism for dealing with it (i.e., a statement that 
>any time tape is 
> removed it is removed completely), and c) that its use 
>had a specific 
> benefit or that there was no good alternative, then 
>using tape would be 
> fine.  I suggested that the specific benefit was that we 
>had different 
> colors for different lab members allowing us to notify 
>the correct person 
> if there is a problem with a tank - and she thought this 
>sounded great 
> (especially as the lab expands towards a dozen members). 
> I mentioned that 
> the only alternative is static labels, but that they 
>fall off, and she 
> agreed that if there was a chance of this (and thus 
>having to sacrifice 
> tanks because the fish were no longer identifiable), 
>then it would be 
> better for the animals to use tape.
> 
> 4) We have had no rust.
> 
> 5) I would avoid this at all costs.  There are fish 
>facilities with fume 
> hoods in them for experiments which may cause vapors, 
>but I would not paint 
> anything in the room after animals are in there.
> 
> 
> At 10:46 AM 10/6/2006, you wrote:
>>Content-class: urn:content-classes:message
>>Content-Type: multipart/alternative;
>>         boundary="----_=_NextPart_001_01C6E956.256F3989"
>>
>>Hi everyone,
>>
>>Our fish facility at Princeton is under my supervision 
>>and animal care has 
>>minimal input into our facility.  The only thing they do 
>>is take the trash 
>>out that we place in the hallway.  However, each time we 
>>come up for 
>>inspection by the University Committee on Animal Care or 
>>larger 
>>inspections from the AAALAC, we begin to have some 
>>disagreements with the 
>>departmental animal care staff.
>>
>>I would like to hear how other fish facilities are run 
>>with respect to 
>>animal care and proper AAALAC practices.  In addition, if 
>>anyone has 
>>answers to the questions below, I would appreciate it.
>>
>>1)       Does a University Animal Care group run your 
>>facility?  If not, 
>>how much input do they provide?
>>2)       Are you required to wear disposable lab coats, 
>>booties, and/or 
>>face masks to enter your facility?
>>3)       What do you label your tanks with?  Does anyone 
>>else use tape? Is 
>>this an issue?
>>4)       How do you deal with rust in your facilities?
>>5)       How do people feel about using paint or other 
>>chemicals that 
>>leave fumes in the facility?
>>
>>
>>Any input would be greatly appreciated!
>>
>>Becky
>>---------------------------------------------------
>>Rebecca D. Burdine, Ph.D.
>>Assistant Professor
>>Dept. of Molecular Biology
>>Princeton University
>>Washington Road Mof 433
>>Princeton, NJ 08544
>>
>>Phone: (609) 258-7515
>>Fax: (609) 258-1343
>>Email: 
>><mailto:rburdine from princeton.edu>rburdine from princeton.edu
>>Admin Assistant: Cathy Falk (609) 258-1604
>>
>>_______________________________________________
>>Zbrafish mailing list
>>Zbrafish from net.bio.net
>>http://www.bio.net/biomail/listinfo/zbrafish
> 
> Thomas Bartman, M.D., Ph.D.
> Divisions of Neonatology, Pulmonary Biology, and 
>Developmental Biology
> Cincinnati Children's Hospital Medical Center
> 3333 Burnet Ave, MLC 7009
> Cincinnati, OH  45229-3039
> 
> Office: 513-636-9902 
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><((((º>`·.¸><((((º>`·.¸><((((º>`·.¸><((((º>
Tiago Filipe Baptista da Rosa Repolho
Departamento de Biologia
Universidade de Aveiro
Campus de Santiago
3810 - 193
Aveiro
Portugal
e-mail: trepolho from bio.ua.pt
web: http://www.bio.ua.pt/ZebraLab_UA_Webpage/Zebralab.htm
><((((º>`·.¸><((((º>`·.¸><((((º>`·.¸><((((º>



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