[Zbrafish] Re: Zbrafish Digest, Vol 17, Issue 4

[John Kanki]

Hi all, I have two topics that I'd like to get input on from the field.

1) Can anyone send me a working protocol for doing double fluorescent 
wholemount insitu preps that really works reliably and well? 
(tyramide-based, etc).

2) We are in the market for replacing our high speed, commercial dish 
washers in the zefish facility, and currently have some made by 
Jackson. Unfortunately these are putting out a dark metallic residue 
due to the reactivity of internal parts with the RO water that we use 
(despite their retrofitting them to be RO-water safe). Does anyone 
know of commercial models that work well for our fish tank purposes 
or that have separate water inputs for the wash and rinse, so that 
the final rinse alone uses RO water? This may prevent the problem we 
see with our current models.
Thanks very much!

John Kanki
-- 



John P. Kanki   PhD
Instructor of Pediatric Oncology
DFCI Zebrafish Project Supervisor
Dana-Farber Cancer Institute
44 Binney St.
Boston, MA  02115

Phone: 617-632-4476
Fax:  617-632-3546
Email:  john_kanki from dfci.harvard.edu