[Zbrafish] embryo contamination
antiquemaniac47906 from yahoo.com
via zbrafish%40net.bio.net
(by antiquemaniac47906 from yahoo.com)
Wed Apr 4 11:18:51 EST 2007
> We are experiencing contamination inside the chorion of our zebrafish
embryos. A 10% system water change occurs 3-4X per week and water
quality tests show normal ranges. The
fertilized eggs are washed with .003% bleach within 20 minutes of
fertilization. Our breeders are about 1 year old. The embryo media
is changed
every 24 hours.
>
>
> The contaminant appears as a cloudy white, flocculent mass which
increases over time until the embryo dies. Does anybody have any
suggestions as
to how we may combat this issue.
>
>
> Thanks for your help,
>
> David Southern - Jim Marrs Lab IUPUI
>
> Don Ratcliffe - Jim Marrs Lab IUPUI
>
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