[Zbrafish] Re: Looking for a transparent zebrafish strain...

eas from stowers-institute.org via zbrafish%40net.bio.net (by eas from stowers-institute.org)
Mon Feb 12 10:25:44 EST 2007


Perhaps this will help:
http://www.popularmechanics.com/technology/industry/1287511.html


Christian Lawrence wrote:
> Lasse -
> I know nothing about this tra fish, but we have a transparent line called
> ruby.  It is a double mutant, homozygous for both roy (no iridophores) and
> albino, so it only functionally expresses the xanthophores and is largely
> transparent.  They are a bit more difficult to raise than albino (they don't
> see as well and do best with dense infusoria early through metamorphosis),
> but once you get them to the juvenile stage, they are relatively  normal  in
> terms of their husbandry requirements.  They must be raised with other
> rubies only; wild-types, roys, and albino will all outcompete them during
> rapid growth phase and maturation. Nearly impossible to raise if your larval
> rearing protocols are not good.  Adults breed normally and can be kept with
> wt with no adverse effects.
>
> The roy fish are also mostly transparent, with the notable exception of the
> eyes (with functional melanocytes and no expressing iridophores, the eyes
> are black, hence the name ROY Orbison).  If you are looking at other
> structures, however, they may be suitable.  They are very easy to rear (read
> = even poorly designed/executed larval rearing protocols will work). Again,
> these fish are virtually the same as wild-type in terms of their husbandry,
> although they will be outcompeted if reared together with wt (prior to
> sexual maturity).   Once they are adults, they can be kept with wt, and they
> (at least our fish) are prodigious spawners.
>
> I don't believe either roy or ruby is available from ZIRC, but we can send
> you roys if you wish.
>
> Good luck,
> Chris
>
>
>
>
>
> On 11 Jan 2007 11:09:01 -0800, Lasse <lasse from scienceshow.dk> wrote:
> >
> > Hello everybody.
> >
> > I have seen in some old publications that there once was a transparent
> > zebrafish strain, originating from the Streissinger Lab, called tra
> > (for transparent). The description of the phenotype is however quite
> > vague. It is said to have the appearance of a transparent body and huge
> > black eyes. It should be fully viable and probably fertile.
> >
> > I was hoping some of you may have some fish of this strain and can tell
> > me a little bit more about it. I will especially appreciate information
> > about the level of transparency (can one for example see the spine
> > clearly through the body?) the fertility (can they be bred just as
> > easily as normal zebrafish) and if the huge eyes has any influence on
> > the percepted stress by the fish (ie if the fish is blind it may not be
> > possible to chase-stress it).
> >
> > I really hope someone on this forum can help me!! Thanks a million in
> > advance!!!
> >
> > Best regards: -Lasse
> >
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> >
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> --
> Christian Lawrence
> Brigham and Women's Hospital
> Karp Family Research Laboratories, 06-004B
> One Blackfan Circle
> Boston, MA 02115
> Tel: 617.355.9041
> Fax: 617.355.9064
>
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> Lasse -<br>I know nothing about this tra fish, but we have a transparent line called ruby.&nbsp; It is a double mutant, homozygous for both roy (no iridophores) and albino, so it only functionally expresses the xanthophores and is largely transparent.&nbsp; They are a bit more difficult to raise than albino (they don&#39;t see as well and do best with dense infusoria early through metamorphosis), but once you get them to the juvenile stage, they are relatively&nbsp; normal&nbsp; in terms of their husbandry requirements.&nbsp; They must be raised with other rubies only; wild-types, roys, and albino will all outcompete them during rapid growth phase and maturation. Nearly impossible to raise if your larval rearing protocols are not good.&nbsp; Adults breed normally and can be kept with wt with no adverse effects.
> <br><br>The roy fish are also mostly transparent, with the notable exception of the eyes (with functional melanocytes and no expressing iridophores, the eyes are black, hence the name ROY Orbison).&nbsp; If you are looking at other structures, however, they may be suitable.&nbsp; They are very easy to rear (read = even poorly designed/executed larval rearing protocols will work). Again, these fish are virtually the same as wild-type in terms of their husbandry, although they will be outcompeted if reared together with wt (prior to sexual maturity).&nbsp;&nbsp; Once they are adults, they can be kept with wt, and they (at least our fish) are prodigious spawners.
> <br><br>I don&#39;t believe either roy or ruby is available from ZIRC, but we can send you roys if you wish.<br><br>Good luck,<br>Chris<br><br><br><br><br><br><div><span class="gmail_quote">On 11 Jan 2007 11:09:01 -0800,
> <b class="gmail_sendername">Lasse</b> &lt;<a href="mailto:lasse from scienceshow.dk">lasse from scienceshow.dk</a>&gt; wrote:</span><blockquote class="gmail_quote" style="border-left: 1px solid rgb(204, 204, 204); margin: 0pt 0pt 0pt 0.8ex; padding-left: 1ex;">
> Hello everybody.<br><br>I have seen in some old publications that there once was a transparent<br>zebrafish strain, originating from the Streissinger Lab, called tra<br>(for transparent). The description of the phenotype is however quite
> <br>vague. It is said to have the appearance of a transparent body and huge<br>black eyes. It should be fully viable and probably fertile.<br><br>I was hoping some of you may have some fish of this strain and can tell<br>
> me a little bit more about it. I will especially appreciate information<br>about the level of transparency (can one for example see the spine<br>clearly through the body?) the fertility (can they be bred just as<br>easily as normal zebrafish) and if the huge eyes has any influence on
> <br>the percepted stress by the fish (ie if the fish is blind it may not be<br>possible to chase-stress it).<br><br>I really hope someone on this forum can help me!! Thanks a million in<br>advance!!!<br><br>Best regards: -Lasse
> <br><br>_______________________________________________<br>Zbrafish mailing list<br><a href="mailto:Zbrafish from net.bio.net">Zbrafish from net.bio.net</a><br><a href="http://www.bio.net/biomail/listinfo/zbrafish">http://www.bio.net/biomail/listinfo/zbrafish
> </a><br><br><br><br><br>THE INFORMATION TRANSMITTED IN THIS ELECTRONIC COMMUNICATION IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHOM IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL.&nbsp;&nbsp;ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON, THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED.&nbsp;&nbsp;IF YOU RECEIVED THIS INFORMATION IN ERROR, PLEASE CONTACT THE SENDER AND THE PRIVACY OFFICER, AND PROPERLY DISPOSE OF THIS INFORMATION.
> <br><br><br></blockquote></div><br><br clear="all"><br>-- <br>Christian Lawrence<br>Brigham and Women&#39;s Hospital<br>Karp Family Research Laboratories, 06-004B<br>One Blackfan Circle<br>Boston, MA 02115<br>Tel: 617.355.9041
> <br>Fax: 617.355.9064
>
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