[Zbrafish] Re: Collecting embryos from AHAB photoperiod tanks
Cheuk-Chung Tony Ho
(by wind.4ever At gmail.com)
Tue Jan 9 04:10:16 EST 2007
This picture should give you a general idea about the device that I was
talking about. There is a cover that goes on top, which prevents the
fish from entering the trap while I am retriving it from the tank.
Burdine, Rebecca D wrote:
> Hi everyone,
> We've been contemplating making devices such as the ones you are
> describing. Would you be willing to send a picture of your collecting
> device for the AHAB or Marine Biotech tanks?
> Rebecca D. Burdine, Ph.D.
> Assistant Professor
> Dept. of Molecular Biology
> Princeton University
> Washington Road Mof 433
> Princeton, NJ 08544
> Phone: (609) 258-7515
> Fax: (609) 258-1343
> Email: rburdine At princeton.edu
> Admin Assistant: Cathy Falk (609) 258-1604
> -----Original Message-----
> From: zbrafish-bounces At oat.bio.indiana.edu
> [mailto:zbrafish-bounces At oat.bio.indiana.edu] On Behalf Of Cheuk-Chung
> Tony Ho
> Sent: Sunday, January 07, 2007 10:07 PM
> To: bionet-organisms-zebrafish At moderators.isc.org
> Subject: [Zbrafish] Re: Collecting embryos from AHAB photoperiod tanks
> I wonder what size is the AHAB tank you are using? My insitution uses
> the Marine Biotech Z-mod, which is slightly different from the AHAB. I
> think the most difficult part is the space limitation in these systems,
> I have seen others having a easier time with collection using
> stand-alone 30 liters tanks. Anyhow, I will shared my experience and
> see if anyone have a better way of doing this.
> I use 4 liters tanks to house three mating pairs and I submerges a
> small collection device (size about 2" x 3" x2") with a hotizontal
> screen to collect embryos. the collection device sits about 1"-2" below
> the surface of water. For colection in the morning, I placed a cover
> over the collection device and take it out of the water carefully.
> Advise Welcome.
> jason.cockington At gmail.com wrote:
> > Hi all,
> > just out of curiousity, how do people collect embryos out of their
> > photoperiod tanks??
> > I just ask, because since we upgraded from our old, home made system,
> > to our new AHAB unit, we have been finding collection a fiddlly
> > process.
> > We place a tray about 5cm deep with marbles in it (2 marbles deep)
> > This works fine, except, when we go to get the trays out of the tanks,
> > the fish either swim into the marbles and get trapped, or there will
> > some kamakazi fish who jump out of the tank entirely.
> > i am just curious to hear if anyone else has come across a similar
> > thing, and if you have, how you went about minimizing hassle.
> > one possible solution i thought of was getting some slime line trays,
> > that would hold 2 layers of marbles but no deeper, thus decreasing the
> > space restriction.
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