[Zbrafish] Looking for a transparent zebrafish strain...
(by clawrence At rics.bwh.harvard.edu)
Thu Jan 11 20:55:48 EST 2007
I know nothing about this tra fish, but we have a transparent line called
ruby. It is a double mutant, homozygous for both roy (no iridophores) and
albino, so it only functionally expresses the xanthophores and is largely
transparent. They are a bit more difficult to raise than albino (they don't
see as well and do best with dense infusoria early through metamorphosis),
but once you get them to the juvenile stage, they are relatively normal in
terms of their husbandry requirements. They must be raised with other
rubies only; wild-types, roys, and albino will all outcompete them during
rapid growth phase and maturation. Nearly impossible to raise if your larval
rearing protocols are not good. Adults breed normally and can be kept with
wt with no adverse effects.
The roy fish are also mostly transparent, with the notable exception of the
eyes (with functional melanocytes and no expressing iridophores, the eyes
are black, hence the name ROY Orbison). If you are looking at other
structures, however, they may be suitable. They are very easy to rear (read
= even poorly designed/executed larval rearing protocols will work). Again,
these fish are virtually the same as wild-type in terms of their husbandry,
although they will be outcompeted if reared together with wt (prior to
sexual maturity). Once they are adults, they can be kept with wt, and they
(at least our fish) are prodigious spawners.
I don't believe either roy or ruby is available from ZIRC, but we can send
you roys if you wish.
On 11 Jan 2007 11:09:01 -0800, Lasse <lasse At scienceshow.dk> wrote:
> Hello everybody.
> I have seen in some old publications that there once was a transparent
> zebrafish strain, originating from the Streissinger Lab, called tra
> (for transparent). The description of the phenotype is however quite
> vague. It is said to have the appearance of a transparent body and huge
> black eyes. It should be fully viable and probably fertile.
> I was hoping some of you may have some fish of this strain and can tell
> me a little bit more about it. I will especially appreciate information
> about the level of transparency (can one for example see the spine
> clearly through the body?) the fertility (can they be bred just as
> easily as normal zebrafish) and if the huge eyes has any influence on
> the percepted stress by the fish (ie if the fish is blind it may not be
> possible to chase-stress it).
> I really hope someone on this forum can help me!! Thanks a million in
> Best regards: -Lasse
> Zbrafish mailing list
> Zbrafish At net.bio.net
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