[Zbrafish] pBlue-TOPO and X-gal Staining

marc via zbrafish%40net.bio.net (by marc from carl39.freeserve.co.uk)
Wed Mar 14 16:48:36 EST 2007

Has anyone used the pBlue-TOPO (promoterless B-galactosidase
expression vector from Invitorgen) reporter system in zebrafish to
detect for promoter activity?
Or has anyone had any experience with detecting gene expression with
an X-gal assay in whole-mount embryos?

Any help would be great.



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