[Zbrafish] Mass Embryo Rearing
(by jbennett from bio.umass.edu)
Thu May 3 09:12:03 EST 2007
I'm assuming you're asking about mass matings to produce embryos....
We use this system with our wild types and it works out very well.
-We have eight 9L tanks (AHAB system), stocked at around 40-50.
-I try to stock a few more females so it's not quite a 1:1 ratio.
-I also try to stock tanks with fish that aren't all siblings, which
I believe increases amount and length of production.
-The tanks are set up once every two weeks starting Monday night, so
we have embryos Tues-Fri (hence the 8 tanks).
-We do have a few "extra" tanks to set up if there is a need for a
large number of embryos.
-The trays we use are homemade, but basically consist of a frame with
a large mesh covering the top and a small mesh covering the
bottom. We usually attach some artificial grass and insert it at an
angle creating a shallow area.
-These tanks get an extra feeding of dry food (ZIRC recipe) during
the week as well.
-I keep track of the amount laid with a 0-5 scale. 5, being the
best, would mean the tank laid for several hours and produced several
hundred/hour. If you want 50,000 daily you will probably need to set
up more than one of this type/size tank.
-When a tank starts to decline in production, I stock another tank to
replace it. I've had a few tanks produce well for over a year, but
this can vary.
-I will use embryos from these mass matings to raise up and replace
our WT stock, but also raise embryos from pair crosses. Again, since
I combine unrelated fish in these mass mating tanks, and collect eggs
throughout the day/week, I believe this helps avoid
inbreeding...(that's another topic in itself that I think was
-When mutant lines are not producing in pair crosses, I have the lab
set them up in this style and they will lay within one or two
times. (Better odds of compatible couples?)
I hope this helps and isn't too confusing. Feel free to ask if you
have any other questions.
At 03:34 PM 5/2/07, Doreen wrote:
>I am interested to hear of other facilities experience with the mass
>raising of wild type embryos. What worked or didn't work? Volume and
>shape of tanks? Stocking density? Harvesting method? Daily egg
>production achieved? Method of breeder replacement? Our current
>estimate/goal for daily production is 50,000 embryos.
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