[Zbrafish] Re: RNAi in zebrafish

[Jon D.Moulton]

Regarding RNAi in zebrafish, this might be helpful:

Gruber J, Manninga H, Tuschl T, Osborn M, Weber K.
Specific RNAi mediated gene knockdown in zebrafish cell lines.
RNA Biol. 2005 Jul-Sep;2(3):101-5. Epub 2005 Jul 19.

The paper includes data on work in embryos, but they do not claim 
success in embryos with RNAi.
Abstract:
http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=ShowDetailView&TermToSearch=17114924
Open-access paper:
http://www.landesbioscience.com/journals/rnabiology/article/gruberRNA2-3.pdf

Let me know how I can help.

Regards,

  - Jon

     Jon D. Moulton, Ph.D.
     Diagnostics and Special Projects
     GENE TOOLS, LLC
     jmoulton from gene-tools.com
     www.gene-tools.com
     (541) 929-7840 x1201
     http://network.nature.com/group/morpholinos

bdschyth wrote:
> On Sep 18, 4:29 am, Joule <bwj... from gmail.com> wrote:
>   
>> On Jul 24, 11:58 pm, senthil kumar <rse... from yahoo.co.in> wrote:
>>
>>     
>>> Is it possible to do RNAi in Zebrafish. Please send me the references..
>>> Thanks
>>> Rsen
>>>       
>>> ---------------------------------
>>>  Did you know? You can CHAT without downloading messenger.  Click here
>>>       
>> It seems that RNAi does not work in zebrafish .
>>     
> -----------------------------------------------------------------------------------------------
> But - why does it not work in fish? I dont think it is that well
> investigated yet. Most studies have been in zebrafish embryos
> microinjected with long dsRNAs. Remember that not many mammalian
> studies on RNAi mediated gene silencing has been publiched neither.
> Brian Dall Schyth, National Veterinary Institute, Denmark
>
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