[Zbrafish] Zebrafish fixation+process protocol for TUNEL

abrun from hsc.unt.edu via zbrafish%40net.bio.net (by abrun from hsc.unt.edu)
Fri Oct 19 00:09:29 EST 2007


I'm new to this type of animal system. I have to fix some 7 day old
zebrafishes. Have also to section them and do TUNEL staining on the
sections. Has anybody experdise in this area? What is the best
fixative? Do I have to decalcify and how with the least impact so I
can do TUNEL staining.
Thanks for all the help.
Anne-Marie



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