[Zbrafish] Graduate student hands-on lab

Daniel Castranova via zbrafish%40net.bio.net (by castrand from mail.nih.gov)
Wed Oct 24 12:57:03 EST 2007


I have also had people try hand dechorionation using sharp forceps  
and it has gone over pretty well.  You forget how hard dechorionation  
is when you are first learning.

Good luck,


Daniel Castranova
Aquatic Specialist
Charles River Laboratories - Contractor
Unit on Vertebrate Organogenesis
Laboratory of Molecular Genetics, NICHD, NIH
Building 6B, Room 322, 6 Center Drive
Bethesda, MD 20892
castrand from mail.nih.gov

On Oct 24, 2007, at 11:30 AM, Thomas Bartman wrote:

> I've been asked to run a one-hour "hands-on" lab to introduce first  
> year graduate students to the zebrafish model system.  There will  
> be about 16 students, and I can scrape together quite a few  
> microscopes.  However, I'm having trouble thinking of anything that  
> is "hands-on" that will occupy them for that long, but not much  
> longer.  For example, just having them look at embryos would only  
> be interesting for a fwe minutes, but it's not like I can have them  
> do an in situ either!
> Things I've thought of are to mix embryos of 2-3 different stages  
> into one dish and ask them to sort them out, or to mix WT and  
> mutant embryos and do the same.  Does anyone else have creative  
> ideas that would expose them to zebrafish and keep their interest?
> Thanks,
> Tom
> Thomas Bartman, M.D., Ph.D.
> Divisions of Neonatology, Pulmonary Biology, and Developmental Biology
> Cincinnati Children's Hospital Medical Center
> 3333 Burnet Ave, MLC 7009
> Cincinnati, OH  45229-3039
> Office: 513-636-9902
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