[Zbrafish] Graduate student hands-on lab

[David Lains]

Hi Tom 

It could be useful if you spent some time on:
Handling and sorting 
Breeding and sexing 
Egg collecting and cleaning
Stress
Anesthesia/Euthanasia
Fin clipping
General husbandry
Water quality

Best Fishes
David Lains <}}}><
Aquaculturist, Research Assistant
Zebrafish International Resource Center
5274 University of Oregon
Eugene, Or 97403
Email: david from zebrafish.org
pH: (541) 346-6028 ext. 18
fax: (541) 346-6151


-----Original Message-----
From: zbrafish-bounces from oat.bio.indiana.edu
[mailto:zbrafish-bounces from oat.bio.indiana.edu] On Behalf Of Thomas Bartman
Sent: Wednesday, October 24, 2007 8:30 AM
To: Zbrafish from magpie.bio.indiana.edu
Subject: [Zbrafish] Graduate student hands-on lab

I've been asked to run a one-hour "hands-on" lab to introduce first 
year graduate students to the zebrafish model system.  There will be 
about 16 students, and I can scrape together quite a few 
microscopes.  However, I'm having trouble thinking of anything that 
is "hands-on" that will occupy them for that long, but not much 
longer.  For example, just having them look at embryos would only be 
interesting for a fwe minutes, but it's not like I can have them do 
an in situ either!

Things I've thought of are to mix embryos of 2-3 different stages 
into one dish and ask them to sort them out, or to mix WT and mutant 
embryos and do the same.  Does anyone else have creative ideas that 
would expose them to zebrafish and keep their interest?

Thanks,

Tom


Thomas Bartman, M.D., Ph.D.
Divisions of Neonatology, Pulmonary Biology, and Developmental Biology
Cincinnati Children's Hospital Medical Center
3333 Burnet Ave, MLC 7009
Cincinnati, OH  45229-3039

Office: 513-636-9902 

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