[Zbrafish] RE: ossiformation

[Mark Reijnen]

Dear All , 

Can somebody tell me if you can have a delay in development when you
grow up embryos in egg water vs system water . We put 60 embryos in a
petridish the first 4 days and add 400 ml systemwater until day 8. 

How much should the concentration of calcium in egg water to get an
optimal ossification of the early stages ? 

Condition egg water:

1.5 ml stock salt added to 1 liter distilled water.
Temp egg water 26 +/- 0.5 degrees.  

Thanks in advance

Mark Reijnen

-----Original Message-----
From: zbrafish-bounces from oat.bio.indiana.edu
[mailto:zbrafish-bounces from oat.bio.indiana.edu] On Behalf Of
zbrafish-request from oat.bio.indiana.edu
Sent: Thursday, August 23, 2007 5:44 PM
To: Mark Reijnen
Subject: Welcome to the "Zbrafish" mailing list

Welcome to the Zbrafish from net.bio.net mailing list!

To post to this list, send your email to:

  zbrafish from net.bio.net

General information about the mailing list is at:

  http://www.bio.net/biomail/listinfo/zbrafish

If you ever want to unsubscribe or change your options (eg, switch to
or from digest mode, change your password, etc.), visit your
subscription page at:

  http://www.bio.net/biomail/options/zbrafish/m.reijnen%40niob.knaw.nl

You can also make such adjustments via email by sending a message to:

  Zbrafish-request from net.bio.net

with the word `help' in the subject or body (don't include the
quotes), and you will get back a message with instructions.

You must know your password to change your options (including changing
the password, itself) or to unsubscribe.  It is:

  supermax

Normally, Mailman will remind you of your net.bio.net mailing list
passwords once every month, although you can disable this if you
prefer.  This reminder will also include instructions on how to
unsubscribe or change your account options.  There is also a button on
your options page that will email your current password to you.