Zbrafish Digest, Vol 28, Issue 3

raj abi via zbrafish%40net.bio.net (by keviraj from yahoo.com)
Fri Sep 7 14:35:34 EST 2007


Hi Sridevi,
  Depending upon whether you intend to do pair spawning or group spawning,you can use different types of breeding tanks. Plasic tanks of 2 lit capacity with a fitting trap to allow the eggs settle at the bottom will be sufficient for pair spawning where as 8 lit glass tank or fibre glass tank with suitable trap will serve the purpose for group spawning.
  In both cases I would suggest you can make your traps or sieve we can say using plastic or fibre glass to fit the size of the tanks.The point here is, the eggs need to be clearly separated and settled down on the bottom of the tank to save it from being eaten up by the adults,meaning eggs can pass through the sieve while the adults should not.You can attach some plastic leaves or some soft substance to the sieve/trap as they love to lay the eggs around it!!!.............and your breeding tank is ready...
   
  rds
  Rajesh Krishnan
  University of Toronto
  
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Today's Topics:

1. Operon Zebrafish oligo probe set (Abria.Queen from gmail.com)
2. Request information for the Zebrafish breeding tanks (sridevi)
3. RE: Request information for the Zebrafish breeding tanks
(christian lawrence)
4. RE: Request information for the Zebrafish breeding tanks
(Eric Moore)


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Message: 1
Date: Fri, 07 Sep 2007 07:17:26 -0700
From: Abria.Queen from gmail.com
Subject: [Zbrafish] Operon Zebrafish oligo probe set
To: bionet-organisms-zebrafish from net.bio.net
Message-ID: <1189174646.952213.98410 from d55g2000hsg.googlegroups.com>
Content-Type: text/plain; charset="iso-8859-1"

Hi everyone,

Operon is planning the release of a microarray ready probe set for
printing microarrays for Zebrafish. The set includes about 22,000 70
mer probes that cover the entire genome. If you or a colleague are
interested please feel free to contact me for more information.

Thank you,

Abria

Abria.Queen from operon.com
256-704-8159



------------------------------

Message: 2
Date: Fri, 7 Sep 2007 12:15:05 +0100 (BST)
From: sridevi 
Subject: [Zbrafish] Request information for the Zebrafish breeding
tanks
To: zbrafish from magpie.bio.indiana.edu
Message-ID: <548255.66283.qm from web7913.mail.in.yahoo.com>
Content-Type: text/plain; charset="iso-8859-1"

Dear Sir/Madam,

I sridevi from India working as a Junior Research Fellow under Dr. V.Kalarani Head, Associate Professor, Department of Biotechnology, Sri Padmavati Women's University, Tirupati, Chittoor Dist., Andhra Pradesh, India.
We are working on zebrafish. So we need the zebrafish breeding tanks. Please give me the modalities and information for purchase of zebrafish breeding tanks.

With Best Regards,

Sridevi


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Message: 3
Date: Fri, 7 Sep 2007 14:11:43 -0400
From: "christian lawrence" 
Subject: RE: [Zbrafish] Request information for the Zebrafish breeding
tanks
To: "'sridevi'" ,

Message-ID: 
Content-Type: text/plain; charset="us-ascii"

Most suppliers (AHAB, MBI, Tecniplast, etc.) make their own version of
breeding trap. Most of these, as well as others made by laboratory supply
companies, are plastic traps that fit inside plastic tanks. 

In my opinion (based upon my personal experience, data collected in
laboratory settings, and observations of the animals spawning in the wild)
the ones that work best are those that can be "tipped", providing a shallow
to deep gradient within the trap. The fish appear to preferentially spawn
in the shallow areas. If you don't believe me, try it, and pay close
attention to how the fish behave and where most of the eggs are laid.

All cages will work. But those that can be easily tipped work better.
Still my opinion. But a semi-informed one.

With this in mind, we use 2 liter polycarbonate mouse cages from nalgene or
tecniplast, fill it with fish water, and insert the following breeding trap,
tip it, and let the fish do their thing (with proper conditioning, of
course).

http://www.aquaticeco.com/index.cfm/fuseaction/product.detail/iid/3377

Using such an approach, you can probably get all supplies in India (mouse
cages, reasonable facsimile of breeding trap).

Also you can check out
http://www.aquatichabitats.com/breeder_tank_large.htm
http://www.tecniplast.it/aquatic/zebtec.php
http://www.marinebiotech.com/index.php?pid=spawning_vessels
http://www.lpsinc.com/Catalog4.asp?catalog_nu=T233790

Good luck,
Chris



Christian Lawrence
Brigham and Women's Hospital
Karp Family Research Laboratories 06-004B
One Blackfan Circle
Boston, Massachusetts 02115
Tel: 617.355.9041
Fax: 617.355.9064




_____ 

From: zbrafish-bounces from oat.bio.indiana.edu
[mailto:zbrafish-bounces from oat.bio.indiana.edu] On Behalf Of sridevi
Sent: Friday, September 07, 2007 7:15 AM
To: zbrafish from magpie.bio.indiana.edu
Subject: [Zbrafish] Request information for the Zebrafish breeding tanks


Dear Sir/Madam,

I sridevi from India working as a Junior Research Fellow under Dr.
V.Kalarani Head, Associate Professor, Department of Biotechnology, Sri
Padmavati Women's University, Tirupati, Chittoor Dist., Andhra Pradesh,
India.
We are working on zebrafish. So we need the zebrafish breeding tanks. Please
give me the modalities and information for purchase of zebrafish breeding
tanks.

With Best Regards,

Sridevi


_____ 

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Message: 4
Date: Fri, 7 Sep 2007 14:16:20 -0400
From: "Eric Moore" 
Subject: RE: [Zbrafish] Request information for the Zebrafish breeding
tanks
To: "sridevi" ,

Message-ID:

Content-Type: text/plain; charset="us-ascii"

Hello Sridevi,



Marine Biotech offers several different types of breeding tanks. Please
email me directly and I'll send you some information. Thanks, Eric



Eric Moore

Territory Manager

Marine Biotech

54A West Dane St.

Beverly, MA 01915 USA

Toll Free US (888) 624-8265 x 132

International (978) 927-8720 x 132

Cell 978 578-2740

Fax 978 921-0231

www.marinebiotech.com 

________________________________

From: zbrafish-bounces from oat.bio.indiana.edu
[mailto:zbrafish-bounces from oat.bio.indiana.edu] On Behalf Of sridevi
Sent: Friday, September 07, 2007 7:15 AM
To: zbrafish from magpie.bio.indiana.edu
Subject: [Zbrafish] Request information for the Zebrafish breeding tanks



Dear Sir/Madam,



I sridevi from India working as a Junior Research Fellow under Dr.
V.Kalarani Head, Associate Professor, Department of Biotechnology, Sri
Padmavati Women's University, Tirupati, Chittoor Dist., Andhra Pradesh,
India.

We are working on zebrafish. So we need the zebrafish breeding tanks.
Please give me the modalities and information for purchase of zebrafish
breeding tanks.



With Best Regards,



Sridevi



________________________________

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End of Zbrafish Digest, Vol 28, Issue 3
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