[Zbrafish] FW: ossification delay in egg water

Mark Reijnen via zbrafish%40net.bio.net (by m.reijnen from niob.knaw.nl)
Tue Sep 11 01:25:02 EST 2007



-----Original Message-----
From: Mark Reijnen 
Sent: Friday, September 07, 2007 9:37 AM
To: 'zbrafish-bounces from oat.bio.indiana.edu'
Subject: FW: ossification delay in egg water 



-----Original Message-----
From: Mark Reijnen 
Sent: Thursday, September 06, 2007 2:02 PM
To: 'zbrafish from net.bio.net'
Subject: RE: ossiformation 

Dear All , 

Can somebody tell me if you can have a delay in development when you
grow up embryos in egg water vs system water . We put 60 embryos in a
petridish the first 4 days and add 400 ml systemwater until day 8. 

How much should the concentration of calcium in egg water to get an
optimal ossification of the early stages ? 

Condition egg water:

1.5 ml stock salt added to 1 liter distilled water.
Temp egg water 26 +/- 0.5 degrees.  

Thanks in advance

Mark Reijnen

-----Original Message-----
From: zbrafish-bounces from oat.bio.indiana.edu
[mailto:zbrafish-bounces from oat.bio.indiana.edu] On Behalf Of
zbrafish-request from oat.bio.indiana.edu
Sent: Thursday, August 23, 2007 5:44 PM
To: Mark Reijnen
Subject: Welcome to the "Zbrafish" mailing list

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