[Zbrafish] whole mount in situ hybridization

Claudia via zbrafish%40net.bio.net (by claudia_hohn from hotmail.com)
Wed Sep 19 09:55:36 EST 2007

I was wondering if anybody has done in situ hybridization on whole
mount zebrafish embryos using oligonucleotides as probes? I found some
good protocols for the actual ISH procedure but none of them used
oligos as a probe. I would like to use a DIG oligonucleotide tailing
kit to prepare probes. Please let me know if you have any experience
with this in zebrafish and what size probe worked best for you.
Thank you,

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