[Zbrafish] in vitro maturation

AJ via zbrafish%40net.bio.net (by uphoryx from gmail.com)
Sat Sep 22 12:09:30 EST 2007


i have been trying to do some invitro maturation using 0.49-0.58mm
oocytes. i am using leibovitz medium. i've incubated the oocytes for 6
hours in hCG and thence with DHP for 10 hours. i am following a
journal, but i can't get any positive results. anyone out there who
can help? thanks.



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