[Zbrafish] Re: Death at 50% Epiboly

[Olivier]

On Sep 19, 10:49 pm, Leviathan <jason.cocking... from gmail.com> wrote:
> HI everyone,
>
> i was just wondering if anyone has ever encountered this before, or
> would have some ideas as to what might be causing it?
>
> I have recently had some of our zebrafish users report a strange die
> off effect in their embryo collections.
>
> When said users collect embryos from 2 of our 3 light cycle units,
> they have been noticing  a rather large die off of around 50%
> occurring somewhere around the 50% epiboly stage.
>
> because it appears to only be effecting collections from 2 of our 3
> breeding systems it has got me stumped.
>
> the water conditions have been pretty stable in these systems:
> oC        28.5
> mS/cm  1.1
> DOsat   20%
> pH        7.0
>
> admittedly, the salinity is a little higher than ideal, but the
> embryos are being transferred to E3 medium after collection, so i
> would have thought if the water was to blame it would have effected
> the embryos earlier!?!
>
> Can anyone shed some light on this for me?
>
> many thanks,
>
> jason

Hi Jason,
Did you check if these fishs have problems inflating their swim
bladders? That could explain why they are dying after 5 days.

Olivier