[Zbrafish] Autofluorescence

123 via zbrafish%40net.bio.net (by gottalovelife from gmail.com)
Tue Apr 1 14:02:42 EST 2008


I'm having issues with autofluorescence interfering with my
fluorescent and confocal imaging.  Does anyone know at which
wavelengths the fish will autofluoresce the least?  I would like to be
able to do my imaging on 4%PFA fixed fish, but I can do it live as
well.  I've already tried sodium borohydride but this does not quench
the autofluorescence adequately.



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