[Zbrafish] Re: Breeding Problems

[Wilson Clements]

> Dear Drs. Khodabakhsh and Mei,
>
> With respect to your breeding problems, we went through similar low  
> fecundity here and tried numerous things.  We found that the thing  
> that made the greatest difference was feeding.  Here was our  
> situation:
>
> Fish were optimal age (6 mos. to 1.2 years old).  Water quality was  
> good, with pH running around 7.1 to 7.2 and conductivity at around  
> 400 to 600 uS.  We tested for nitrites, nitrates, and ammonia and  
> found our levels to be reasonable.  Still we were having fertility  
> issues.
>
> We changed our feeding schedule to have two brine shrimp feedings  
> per day with 1 liter of brine shrimp per rack, hatched at 6g of  
> cysts per liter.  On week days, individuals feed their racks with  
> an evening supplemental feeding of "Scientific Hatcheries 3 Pigment  
> Diet/ Fish Food for Zebrafish/Danio Rerio"  Product # SFF1 from  
> Aquaneering, 7960 Stromesa Ct., San Diego, CA, 92126, USA  
> 858-578-2028.  I generally feed my fish enough food that they can  
> clear the top surface of the water in approximately 2 minutes.
>
> Since we have adopted this feeding routine, 60-90% of my crosses  
> are productive.
>
> Here are additional things we tried that seem to make some limited  
> difference:
>
> 1) Change water in the morning prior to breeding.
> 2) Shield fish from room activity (but not light!).
> 3) Marbles and/or fake seaweed in the breeding chamber.
> 4) Angle breeding chambers to create a "shallow" end.
>
> I also read that providing new water with slightly lower  
> conductivity (~200-300 uS) in the morning of breeding helped.  This  
> was not a feasible option for us, so we have never tried it, and I  
> don't know if it works.
>
> Hope this helps.
>
> Best,
> Wilson
> ---------------------------------------------------------------------- 
> ----------------------
> Wilson Clements, Ph.D.
>
> wclements from ucsd.edu
>
> Dept. of Biology
> Section of Cell and Developmental Biology
> University of California at San Diego
> 9500 Gilman Dr.
> Natural Sciences Building 6105
> La Jolla, CA 92093-0380
>
> TEL    (858) 534-6955
> LAB    (858) 822-4658
> FAX    (858) 822-5740
>
>
> Begin forwarded message:
>> Today's Topics:
>>
>>    1. UCSF Mission Bay - Guo Lab (Hadie Khodabakhsh)
>>    2. Breeding zebrafish (Wenyan Mei)
>>
>>
>> --------------------------------------------------------------------- 
>> -
>>
>> Message: 1
>> Date: Wed, 9 Apr 2008 13:42:43 -0700 (PDT)
>> From: Hadie Khodabakhsh <hadie_k1984 from yahoo.com>
>> Subject: [Zbrafish] UCSF Mission Bay - Guo Lab
>> To: zbrafish from magpie.bio.indiana.edu
>> Message-ID: <469021.20978.qm from web35505.mail.mud.yahoo.com>
>> Content-Type: text/plain; charset="iso-8859-1"
>>
>> Good afternoon,
>>
>> I was wondering if you had any suggestions, or methods that will  
>> get zebrafish to mate more. I have already placed my zebrafish in  
>> the best environmental conditions, but they just don't seem to  
>> mate. So I was wondering if there were any hormonal treatment  
>> methods that can be used to influence the zebrafish to mate more?
>>
>> Thank you,
>>
>> Hadie Khodabakhsh
>>
>> (In case you are wondering, I am a research associate at the  
>> zebrafish lab of Dr. Guo at UCSF Mission Bay)
>>
>>  __________________________________________________
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>> ------------------------------
>>
>> Message: 2
>> Date: Wed, 09 Apr 2008 10:36:38 -0400
>> From: Wenyan Mei <meiw from ccri.net>
>> Subject: [Zbrafish] Breeding zebrafish
>> To: <zbrafish from magpie.bio.indiana.edu>
>> Message-ID: <C4224CB6.1D5A%meiw from ccri.net>
>> Content-Type: text/plain;	charset="US-ASCII"
>>
>> Dear Jen,
>>
>> I am an investigator at the Ohio State University. We are having  
>> big problem
>> with our fish breeding. It has been two and half years since my fish
>> facility was built by Aquatic Habitats. The water condition is  
>> good in our
>> fish facility: pH around 7.3-7.6, Conductivity around 250-300, and
>> Temperature is a little higher (29C). We feed brine shrimp twice  
>> daily for
>> the fish. Females look great with big belly. But they simply don't  
>> lay eggs
>> for us. This really bother us because our project progress is  
>> delayed due to
>> breeding problem. I am wondering if you can give me some  
>> suggestions to make
>> fish breed better. Thanks a lot.
>> wenyan
>

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