[Zbrafish] Zebrafish Age / Size Variations;
(by Christian.Lawrence from childrens.harvard.edu)
Fri Aug 8 11:54:41 EST 2008
FYI: For discussion of relationships between between sexual
differentiation, body length, and age, see: Maak H, Segner CR, and Tyler R
(2003) Ontogeny of sexual differentiation in different strains of zebrafish
(Danio rerio). Fish Physiology and Biochemistry, Volume 28, Numbers 1-4,
2003 , pp. 125-128(4).
On 8/8/08 7:12 AM, "Philippe Herbomel" <herbomel from pasteur.fr> wrote:
> Sorry, please replace my previous message (in which the reference of the book
> wasn't quite accurate)
> by this one.
> Philippe Herbomel
> Dear Oliver,
> Body length appears indeed to be the adequate, simple parameter for estimating
> postembryonic developmental stage.
> Our reference in this matter is Tom Schilling's excellent chapter on "The
> morphology of larval and adult zebrafish" (pp. 59-94 In "Zebrafish: A
> Practical Approach." 2002, eds. C. Nusslein-Volhard and R. Dahm, Oxford Univ.
> Press), in which Table 2 provides a typical correspondence between "age" and
> body length" up to 90 days.
> In their study of the development and maturation of the immune system in
> zebrafish, Lam et al. ( Dev. Compar. Immunol. 28 : 9-28, 2004) also described
> this maturation relative not only to age but also to body length (see
> Discussion), which is obviously more significant and helpful. You will see
> that they give the same correspondence between age and body length as Tom
> Schilling at 2 weeks but not at 4 weeks.
> Interestingly, an older paper comparing the role of thyroid hormone in
> zebrafish (vs. axolotl) metamorphosis, i.e. their gradual transformation form
> larvae into juveniles, also used body length to express the developmental
> stages (Brown D., PNAS 94 : 13011-16, 1997).
> This is obviously an important issue, and certainly all zebrafish studies
> dealing with stages past the first week of development should refer to body
> length in addition to or instead of nominal age.
> Also, the community should agree about how to measure that length. In Figure 1
> and Table 2 of his chapter refered above, Tom Schilling provided two different
> measurements : 'Full length' i.e. up to the tip of the caudal fin, and
> 'standard length', ie up to the base of the caudal fin.
> I wasn't at the last Madison meeting but since there was a workshop there
> about "working with post-embryonic stages", maybe this staging issue was
> raised ? I would be interested to learn about this from participants to this
> Best wishes,
> Philippe Herbomel
> Unite Macrophages et Developpement de l'Immunite
> Departement de Biologie du Developpement
> Institut Pasteur
> 25 rue du Dr Roux, 75724 Paris cedex 15, France
> tel. : 33 1 44 38 95 29 mobile: 33 6 73 35 74 20
> fax : 33 1 45 68 89 21
> Change the world one loan at a time - visit Kiva.org to find out how
> Le 7 août 08 à 21:02, Oliver Braubach a écrit :
>> Dear All.
>> I have been working on aspects of postembryonic development in the zebrafish
>> brain, and have been trying to determine a standard method for 'aging'
>> zebrafish. As many of you may be aware, the development of individual fish
>> varies greatly during the first month of development, which ultimately leads
>> to very noticeable differences in the size of individual fish and their
>> Thus, age does not accurately characterize developmental stages of fish, and
>> I have been trying to determine if there's another standard method to do so.
>> Has anyone tried to correlate body length to fish age? Are there any
>> citations for expected / optimal size of fish are a certain age?
>> Any input regarding this problem would be greatly appreciated.
>> Oliver Braubach
>> Department of Physiology and Biophysics
>> Dalhousie University
>> Halifax, NS B3H 1X5 Canada
>> Tel: 902.494.6471
>> Fax: 902.494.1685
>> Zbrafish mailing list
>> Zbrafish from net.bio.net
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