[Zbrafish] purchased hanks for squeezing?

Daniel Castranova via zbrafish%40net.bio.net (by castrand from mail.nih.gov)
Wed Aug 13 08:54:07 EST 2008


Jocelyn,

I have not used Gibco's hanks for IVF in zebrafish but many short  
term extenders used in other fish species include glucose.  I don't  
think the slightly higher osmolarity will be a problem since high  
osmolarity sequesters sperm activation.

I'd give it a shot.


Good luck,

Dan



Daniel Castranova
Aquatic Specialist
Charles River Laboratories - Contractor
Unit on Vertebrate Organogenesis
Laboratory of Molecular Genetics, NICHD, NIH
Building 6B, Room 322, 6 Center Drive
Bethesda, MD 20892
(301)594-0904
castrand from mail.nih.gov


On Aug 12, 2008, at 2:21 PM, Jocelyn wrote:

> Has anyone used Gibco's Hanks in an invitro fertilization protocol?
>
> I'm noticing that Gibco's formula includes glucose (to up the
> osmolarity?), where as the Zebrafish book recipe does not use glucose.
> Will this affect the viability of the sperm?
>
> I need to do a squeeze, and don't have the stock solution components
> on hand to make hanks, but instead have the Gibco premade 10x hanks (-
> bicarb, - phenol red).
>
> Any advice?
>
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