[Zbrafish] purchased hanks for squeezing?
(by castrand from mail.nih.gov)
Wed Aug 13 08:54:07 EST 2008
I have not used Gibco's hanks for IVF in zebrafish but many short
term extenders used in other fish species include glucose. I don't
think the slightly higher osmolarity will be a problem since high
osmolarity sequesters sperm activation.
I'd give it a shot.
Charles River Laboratories - Contractor
Unit on Vertebrate Organogenesis
Laboratory of Molecular Genetics, NICHD, NIH
Building 6B, Room 322, 6 Center Drive
Bethesda, MD 20892
castrand from mail.nih.gov
On Aug 12, 2008, at 2:21 PM, Jocelyn wrote:
> Has anyone used Gibco's Hanks in an invitro fertilization protocol?
> I'm noticing that Gibco's formula includes glucose (to up the
> osmolarity?), where as the Zebrafish book recipe does not use glucose.
> Will this affect the viability of the sperm?
> I need to do a squeeze, and don't have the stock solution components
> on hand to make hanks, but instead have the Gibco premade 10x hanks (-
> bicarb, - phenol red).
> Any advice?
> Zbrafish mailing list
> Zbrafish from net.bio.net
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