[Zbrafish] Re: seasonal mortality?

Burdine, Rebecca D via zbrafish%40net.bio.net (by rburdine from Princeton.EDU)
Thu Dec 11 09:48:58 EST 2008

Our system is purely RO and we aren't seeing this seasonal issue.  I would suggest checking on two things.  First, when does the RO system get serviced?  Is there a connection between servicing and your seasonal issue?  Second are you monitoring room temp?  Our facility is in an older building and it takes forever for the temperature to even itself out, even in temp maintained rooms.  Is it possible that you are having a severe temp spike (up or down) in the fall?
Rebecca D. Burdine, Ph.D.
Assistant Professor
Dept. of Molecular Biology
Princeton University
Washington Road Mof 433
Princeton, NJ 08544 
Phone: (609) 258-7515
Fax: (609) 258-1343
Email: rburdine from princeton.edu
Admin Assistant: Cathy Falk (609) 258-1604


From: zbrafish-bounces from oat.bio.indiana.edu on behalf of David G. White
Sent: Wed 12/10/2008 6:57 PM
To: Jocelyn
Cc: bionet-organisms-zebrafish from moderators.isc.org
Subject: Re: [Zbrafish] Re: seasonal mortality?

Hello, if your on a purely RO water supply I would guess not. That system would just have a higher reject volume, water not passing through the membrane. You might notice this if your RO pressure or volume is lower than normal, unless you have ample capacity. With a purely DI system yes, it does not reject water and only removes ions in the water. Water will pass through the resin of a DI system regardless of quality (even if the resin is spent). DI is more common then RO water in my experience.

Sounds like you are on a centralized system. I would contact whomever services the system. It might require more frequent maintenance during the fall, carbon filters, resin or membranes.

David G White
Research Coordinator
H225 Zebrafish Lab
University of Washington
Department of Biological Structure
HSB G514 Box 357420
1959 NE Pacific Street
Seattle, WA  98195-7420
Tel. 206-685-7512
FAX 206-543-1524

On Wed, 10 Dec 2008, Jocelyn wrote:

> Off list I received three emails indicating a shared experience of
> increased nursery mortality in fall.
> More about our parameters:
> + we have an RO/DI water source.
> + we have consistent personnel year round (ie full time staff, no
> student workers).
> + we feed powdered AP diet and raise brine shrimp for our nursery fish
> (no paramecia, rotifers, etc ).
> + another zebrafish facility in the same building reported anecdotally
> about similar problems at the same time.
> Will seasonal changes in city water persist through a RO/DI system?
> I'm coming to think so?
> thanks for your feedback.
> As always, its been interesting.
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