[Zbrafish] Help with injected intron containing dsRed mRNA

xiawei via zbrafish%40net.bio.net (by xiaw from umbi.umd.edu)
Fri Feb 1 14:09:54 EST 2008


I'm planning to generate some RNAs which was transcribed from pCS2+  
plasmid using Ambion mMESSAGEmMACHINE kit  and  contains a intron  
inserted dsRed sequence. Does anybody know if the injected intron  
containg RNA can be processed into mature dsRed mRNA and give a  
normal expression of red fluorescence?
Thanks very much.
Wei



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