[Zbrafish] Dechorionation of zebrafish embryos

[prajakta dongre]

Dear all

I want to dechorionate zebrafish embryos by pronase
treatment. I have some questions regarding the same.
Following are the questions.
1) Is pronase and proteinase K same? 
2) From where pronase can be order, Is it Sigma or
Merck or which company?
3) How is pronase stock prepared, is it dissolved in
water or which buffer of what pH?
4) What is the working concentration of pronase for
dechorionation?
5) Any other suggestions regarding dechorionation is
welcome.
Thanking you
Yours sincerely
Prajakta Limaye


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