From vinagre.tania from gmail.com Thu Jan 3 12:33:34 2008 From: vinagre.tania from gmail.com (Tania Vinagre) Date: Thu Jan 3 12:45:14 2008 Subject: [Zbrafish] PSM Promoters Message-ID: <45fdf4b00801030933lb12d228h44db81a96e6c5345@mail.gmail.com> Hi! I am looking for a good zebrafish presomitic mesoderm-specific promoter. Does any one know any good one? Many thanks in advance. Tania -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/zbrafish/attachments/20080103/2e1f0926/attachment.html From vinagre.tania from gmail.com Mon Jan 7 05:15:32 2008 From: vinagre.tania from gmail.com (Tania Vinagre) Date: Mon Jan 7 12:54:51 2008 Subject: [Zbrafish] PSM Promoters Message-ID: <45fdf4b00801070215w13d0f252l526490d45ffd6119@mail.gmail.com> Hi, I am looking for zebrafish promoters that drive expression in the Presomitic Mesoderm.Can anyone suggest me a good one? Many thanks Tania -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/zbrafish/attachments/20080107/69c88429/attachment.html From zfmeeting from gmail.com Mon Jan 7 08:55:14 2008 From: zfmeeting from gmail.com (Zebrafish Meeting) Date: Mon Jan 7 12:55:14 2008 Subject: [Zbrafish] suggestions for workshops at 2008 meeting Message-ID: <3b9058c00801070555y7636610fm87b0732fc43ef074@mail.gmail.com> Dear zebrafish community, Happy new year to everyone. We are working hard on planning for the 2008 international zebrafish meeting (June 25-29, Madison), and would like to solicit your help. This year's meeting will introduce *workshops* on specialized topics proposed by the community. These will be organized in two sessions, each with up to 5 workshops running concurrently. Workshops will last two hours, and might consist of 3 or 4 short (e.g. 15 min) talks, with extensive time for discussion. Our goal is to have a more free-form format in which to discuss emerging, specialized, or controversial topics. Sample topics could be: -- a specialized scientific topic -- an emerging technique -- a challenge facing the community We are now asking for the community to propose the topics for these workshops. Topics will be posted on a blog: http://zf2008workshops.blogspot.com/ and we encourage everyone in the community to visit this blog and comment on proposals. To propose a topic, email zfmeeting@gmail.com. Please include your name and institution. We will invite you to be a blog *author* so that you can describe your proposal in a new post. If you want to comment on a topic, or propose a change, simply *comment* on the relevant post (please include your name and institution in your comment). Our plan is to select the best 10 topics suggested, and then select organizers for each workshop, who will help to solicit talks and also choose talks from submitted abstracts. So be warned: if you suggest a good topic, you may end up organizing the workshop! The blog is up as of today. We will accept new topics through *25 Jan 2008*, so please look very soon. The blog will remain open for comments through 1 Feb 2008, when we will choose a final list of workshop topics. --The 2008 meeting organizers: Sharon Amacher Chi-Bin Chien Laure Bally-Cuif Matthias Hammerschmidt Koichi Kawakami Brant Weinstein -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/zbrafish/attachments/20080107/647d4135/attachment.html From bozena.polok from irovision.ch Tue Jan 8 01:20:19 2008 From: bozena.polok from irovision.ch (Bozena) Date: Tue Jan 8 12:39:27 2008 Subject: [Zbrafish] Swiss Zebrafish Meeting Reminder Message-ID: <22c102ff-a31c-4285-84a7-27e5b594cea0@v67g2000hse.googlegroups.com> Dear Colleagues: This is the reminder for the Ist Swiss Zebrafish Meeting, taking place 14-15th March 2008 in Veysonnaz, Switzerland The keynote speakers are Markus Affolter and Jochen Wittbrodt. Dealine for registration is 15th of January. Please for more information check our webpage www.swisszebra.ch See you there! Best, Bozena Polok IRO Zeb-IRO Av.Gd.Champsec 64 1950 Sion Switzerland +41 27 205 79 06 +41 27 205 79 01 From bobbi from aquaneering.com Mon Jan 14 15:30:58 2008 From: bobbi from aquaneering.com (Bobbi/Aquaneering Inc.) Date: Mon Jan 14 15:33:57 2008 Subject: [Zbrafish] FW: Zebrafish Workshop at WAS Message-ID: <002001c856ec$6251fcb0$6b01a8c0@RoryOffice> Skipped content of type multipart/alternative-------------- next part -------------- A non-text attachment was scrubbed... Name: WAS Zebrafish Workshop Flyer.pdf Type: application/pdf Size: 573156 bytes Desc: not available Url : http://www.bio.net/bionet/mm/zbrafish/attachments/20080114/24fcb46a/WASZebrafishWorkshopFlyer-0001.pdf From Mike.Kacergis from gmail.com Wed Jan 16 11:14:45 2008 From: Mike.Kacergis from gmail.com (Mike.Kacergis@gmail.com) Date: Wed Jan 16 14:50:09 2008 Subject: [Zbrafish] Thought I'd pass along bulb pricing Message-ID: <43974630-240f-44e5-888f-3ba856c77d92@e23g2000prf.googlegroups.com> I came across a source of bulbs that are about 1/3 the price of the large scientific suppliers. An example is the bulb for our fluorescent scope. It takes an HBO 103 w/2 OSRAM 100W 23V DC 2 electrodes. The manufacturer of the scope was charging us approximately $315 per bulb. The same bulb from Atlanta Light Bulbs is only $117.00 In light of budgetary cut-backs and funding issues here is one supplier worth dealing with. www.atlantalightbulbs.com Atlanta Light Bulbs Inc. 1810 Auger Drive Suite-G Tucker GA 30084 Phone 888-988-2852 (toll free) Fax 678-280-0279 Mike From dongrep from yahoo.com Wed Jan 23 08:28:21 2008 From: dongrep from yahoo.com (prajakta dongre) Date: Wed Jan 23 12:07:16 2008 Subject: [Zbrafish] Abt Food of Zebrafish Message-ID: <565790.83762.qm@web37304.mail.mud.yahoo.com> Dear All Zebrafish Lovers I am student from Indian Institute of Science, Bangalore. I am establishing zebrafish as a model in Dr. Upendra Nongthomba's lab. I have query regarding feeding of zebrafish with Drosophila. Should the fish be fed with etherized Drosophila? Will etherized Drosophila affect Zebrafish Adults as well as Fries? Bye Prajakta Limaye ____________________________________________________________________________________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ From jeedward from yahoo.com Wed Jan 23 13:22:27 2008 From: jeedward from yahoo.com (John Edward) Date: Wed Jan 23 13:23:52 2008 Subject: [Zbrafish] BCBGC-08 Final call for papers Message-ID: <335992.49290.qm@web45913.mail.sp1.yahoo.com> BCBGC-08 Final call for papers The 2008 International Conference on Bioinformatics, Computational Biology, Genomics and Chemoinformatics (BCBGC-08) (website: www.PromoteResearch.org ) will be held during July 7-10 2008 in Orlando, FL, USA. The draft paper submission deadline is February 4 2008. BCBGC brings together both academic and industrial scientists and developers from a diverse range of disciplines including bioinformatics, computer science, computational biology, genomics, proteomics and chemoinformatics. One of the main goals of the conference is to promote the dissemination of research to a multidisciplinary audience and to facilitate communication among researchers in different fields. Papers that demonstrate applications of existing techniques or developments of new methods are equally welcomed to the conference. The conference will be held at the same time and location where some other major events will be taking place. The website contains more details. Sincerely John Edward --------------------------------- Never miss a thing. Make Yahoo your homepage. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/zbrafish/attachments/20080123/8de6e504/attachment.html From s_uchida21 from yahoo.co.jp Thu Jan 24 23:35:04 2008 From: s_uchida21 from yahoo.co.jp (s_uchida21@yahoo.co.jp) Date: Fri Jan 25 12:10:08 2008 Subject: [Zbrafish] Feeding C. elegans to Zebrafish? Message-ID: <530daad1-55f2-4cbb-afbd-acf068382d70@m34g2000hsb.googlegroups.com> Dear All: Hello. I have a question concerning food for zebrafish. For an experimental purpose, I would like to feed c. elegans to zebrafish. Do any of you have feeded c. elegans to your fish? Any comments are welcome. Thank you very much for your help in advance. Shizuka From david from zebrafish.org Fri Jan 25 13:52:24 2008 From: david from zebrafish.org (David Lains) Date: Fri Jan 25 13:54:38 2008 Subject: [Zbrafish] Feeding C. elegans to Zebrafish? In-Reply-To: <530daad1-55f2-4cbb-afbd-acf068382d70@m34g2000hsb.googlegroups.com> References: <530daad1-55f2-4cbb-afbd-acf068382d70@m34g2000hsb.googlegroups.com> Message-ID: <028801c85f83$6ffe4b30$4ffae190$@org> Hello Shizuka Aquarium hobbyists use microworms, Walter worms and vinegar eels as live food for feeding fry. I would assume C elegans would be very similar to these nematodes as food item. Best Fishes David Lains <}}}>< Aquaculturist, Research Assistant Zebrafish International Resource Center 5274 University of Oregon Eugene, Or 97403 Email: david@zebrafish.org pH: (541) 346-6028 ext. 18 fax: (541) 346-6151 -----Original Message----- From: zbrafish-bounces@oat.bio.indiana.edu [mailto:zbrafish-bounces@oat.bio.indiana.edu] On Behalf Of s_uchida21@yahoo.co.jp Sent: Thursday, January 24, 2008 8:35 PM To: bionet-organisms-zebrafish@moderators.isc.org Subject: [Zbrafish] Feeding C. elegans to Zebrafish? Dear All: Hello. I have a question concerning food for zebrafish. For an experimental purpose, I would like to feed c. elegans to zebrafish. Do any of you have feeded c. elegans to your fish? Any comments are welcome. Thank you very much for your help in advance. Shizuka _______________________________________________ Zbrafish mailing list Zbrafish@net.bio.net http://www.bio.net/biomail/listinfo/zbrafish From trevarro from uoneuro.uoregon.edu Fri Jan 25 14:41:18 2008 From: trevarro from uoneuro.uoregon.edu (Bill Trevarrow) Date: Fri Jan 25 14:45:09 2008 Subject: [Zbrafish] Feeding C. elegans to Zebrafish? Message-ID: Hi Shizuka, Like David has said microwrms, Walter worms, and vinegar eels are frequently used by hobbyists. My understanding is they are in different but related genera. I have used both microworms and vinegar eels, to feed larval and small juvenile Zfish. Larger fish will also eat them but they will not be getting as great a nutritional component per bite as they would with larger foods. Before using microworms and vinegar eels, I tried growing some C.elegans I obtained from a lab. I did not have the methods to grow the C. elegans in sufficient quantities to be useful, but if you are able to get sufficient quantities of C. elegans I would expect them to work as well as the microworms. The microworms and vinegar eels can be cultured and collected in gram quantities. I suspect that there are ways to do this with C.elegans that I am not aware of. Large scale microworm culturing methods might work with C. elegans. Here is a link to a method that has work well for me: http://zfin.org/zf_info/zfbook/chapt3/3.8.html I like the vinegar eels better than the microworms because they are aquatic animals (unlike the microworms). They would swim up in the water column, making them a more appealing prey item and they would stay alive for a week. The microworms would squirm around on the tank bottom and die in a few hours. You might be able to make a floating mesh worm feeder, from which the worms would slowly work their way out of and fall to the bottom, presenting themselves as a more obvious prey item in the process. Hello Shizuka Aquarium hobbyists use microworms, Walter worms and vinegar eels as live food for feeding fry. I would assume C elegans would be very similar to these nematodes as food item. Best Fishes David Lains <}}}>< Aquaculturist, Research Assistant Zebrafish International Resource Center 5274 University of Oregon Eugene, Or 97403 Email: david@zebrafish.org pH: (541) 346-6028 ext. 18 fax: (541) 346-6151 -----Original Message----- From: zbrafish-bounces@oat.bio.indiana.edu [mailto:zbrafish-bounces@oat.bio.indiana.edu] On Behalf Of s_uchida21@yahoo.co.jp Sent: Thursday, January 24, 2008 8:35 PM To: bionet-organisms-zebrafish@moderators.isc.org Subject: [Zbrafish] Feeding C. elegans to Zebrafish? Dear All: Hello. I have a question concerning food for zebrafish. For an experimental purpose, I would like to feed c. elegans to zebrafish. Do any of you have feeded c. elegans to your fish? Any comments are welcome. Thank you very much for your help in advance. Shizuka -- Bill ~~~~~~~~~~~~~~~~~~~~~~ ?.??.???`?.?.???`?...?><)))?> . , . .???`?.. ><)))?> ><> <>< ><> <>< ><> <>< ><> <>< Bill Trevarrow Institute of Neuroscience University of Oregon 1254 Eugene, OR 97403-1254 Off. Tel: (541) 346-4598 Cell: (541) 844-9054 Fax: (541) 346-4548 e-mail: trevarro@uoneuro.uoregon.edu -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/zbrafish/attachments/20080125/07e039d5/attachment.html From s_uchida21 from yahoo.co.jp Fri Jan 25 22:59:06 2008 From: s_uchida21 from yahoo.co.jp (s_uchida21@yahoo.co.jp) Date: Mon Jan 28 13:17:01 2008 Subject: [Zbrafish] Re: Feeding C. elegans to Zebrafish? References: Message-ID: <885b3502-4745-4a6d-9559-31c5793ba302@i29g2000prf.googlegroups.com> Thank you very much for all of your comments. I will try to feed C. elegans to zebrafish to see if they eat them or not. From s_uchida21 from yahoo.co.jp Sun Jan 27 15:17:58 2008 From: s_uchida21 from yahoo.co.jp (s_uchida21@yahoo.co.jp) Date: Mon Jan 28 13:17:07 2008 Subject: [Zbrafish] Methyl cellulose Message-ID: Hello. I want to take pictures of live zebrafish embryos. I was introduced to methyl cellulose solution. According to the section "METHYL CELLULOSE MOUNTING" of The Zebrafish Book, methyl cellulose can be purchased from Sigma-Aldrich (catalog number M-0387). As indicated in its product sheet, it is sold as powders and takes about 3 days to dissolve. Are there any products of methyl cellulose that is already in solution and ready-to-use format? Thank you very much for your help in advance. Shizuka From rburdine from Princeton.EDU Mon Jan 28 13:31:24 2008 From: rburdine from Princeton.EDU (Burdine, Rebecca D) Date: Mon Jan 28 13:32:36 2008 Subject: [Zbrafish] Re: Feeding C. elegans to Zebrafish? In-Reply-To: <885b3502-4745-4a6d-9559-31c5793ba302@i29g2000prf.googlegroups.com> References: <885b3502-4745-4a6d-9559-31c5793ba302@i29g2000prf.googlegroups.com> Message-ID: <493D6B118ABD73409A22AFAD3D197D91A473@MBCLUSTER.pu.win.princeton.edu> Just FYI, You can grow C. elegans in spinning flask culture to obtain large amounts of them. I haven't don it myself in a very long time, but I am sure the C. elegans most recent wormbook or their website would have protocols. Becky --------------------------------------------------- Rebecca D. Burdine, Ph.D. Assistant Professor Dept. of Molecular Biology Princeton University Washington Road Mof 433 Princeton, NJ 08544 Phone: (609) 258-7515 Fax: (609) 258-1343 Email: rburdine@princeton.edu Admin Assistant: Cathy Falk (609) 258-1604 > -----Original Message----- > From: zbrafish-bounces@oat.bio.indiana.edu > [mailto:zbrafish-bounces@oat.bio.indiana.edu] On Behalf Of > s_uchida21@yahoo.co.jp > Sent: Friday, January 25, 2008 10:59 PM > To: bionet-organisms-zebrafish@moderators.isc.org > Subject: [Zbrafish] Re: Feeding C. elegans to Zebrafish? > > Thank you very much for all of your comments. I will try to feed C. > elegans to zebrafish to see if they eat them or not. > > _______________________________________________ > Zbrafish mailing list > Zbrafish@net.bio.net > http://www.bio.net/biomail/listinfo/zbrafish > From rene.zimmermann from mpi-bn.mpg.de Thu Jan 31 07:15:20 2008 From: rene.zimmermann from mpi-bn.mpg.de (=?ISO-8859-1?Q?Ren=E9?=) Date: Thu Jan 31 12:23:16 2008 Subject: [Zbrafish] Water chemistry Message-ID: <953120d8-442e-4862-82b6-db4bb323b7b8@v17g2000hsa.googlegroups.com> We may still have a problem with our water chemistry. Does anyone know if 100 ?g/l Zinc is suitable for zebrafish? The company claims that this not a problem. However, fish specialists say it's too much. What do you think? Thanks in advance for your help! From trevarro from uoneuro.uoregon.edu Thu Jan 31 15:13:02 2008 From: trevarro from uoneuro.uoregon.edu (Bill Trevarrow) Date: Thu Jan 31 15:15:01 2008 Subject: [Zbrafish] Water chemistry Message-ID: Hi Rene, There are no simple answers to this. Copper and zinc toxicity are influenced by the hardness/softness of the water. The softer the water, the lower the dose of zinc or copper that can affect your fish. The toxicity varies with the kind of fish and as we all know there is little of this kind of specific information available for zebrafish. Zinc is less toxic than copper, which is good in your case, but your numbers are not that far from levels that can cause problems in some kinds of fish in some situations. You might also want to check for other potential metals in your water and possibly determine where the zinc is coming from. When we had problems, copper and zinc water coming from new copper pipes. it was greater in the hot water lines and jumped up in concentration after going through a non-stainless steel heat exchanger (water heater). We successfully used RO machines to remove copper and zinc from the water we used. Resins to remove the copper and zinc lowered the copper and zinc concentrations but also lowered the water hardness so much that the metal toxicity increased. If you want to do a test, set-up an aquarium with water from a zinc free source, while holding all other factors as similar as possible, and compare relevant results (survival, growth, egg laying, or symptoms listed in the ZIRC link for copper) after a week or two. Some references: Copper: http://zebrafish.org/zirc/health/diseaseManual.php#Copper%20Toxicity Copper and other metals: G. Wedemeyer, Physiology of Fish in Intensive Aquaculture >We may still have a problem with our water chemistry. Does anyone know >if 100 ?g/l Zinc is suitable for zebrafish? >The company claims that this not a problem. However, fish specialists >say it's too much. >What do you think? >Thanks in advance for your help! > >_______________________________________________ >Zbrafish mailing list >Zbrafish@net.bio.net >http://www.bio.net/biomail/listinfo/zbrafish -- Bill ~~~~~~~~~~~~~~~~~~~~~~ ?.??.???`?.?.???`?...?><)))?> . , . .???`?.. ><)))?> ><> <>< ><> <>< ><> <>< ><> <>< Bill Trevarrow Institute of Neuroscience University of Oregon 1254 Eugene, OR 97403-1254 Off. Tel: (541) 346-4598 Cell: (541) 844-9054 Fax: (541) 346-4548 e-mail: trevarro@uoneuro.uoregon.edu -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/zbrafish/attachments/20080131/17ee9387/attachment.html From finchg from ohsu.edu Thu Jan 31 20:47:07 2008 From: finchg from ohsu.edu (finchg@ohsu.edu) Date: Fri Feb 1 12:26:10 2008 Subject: [Zbrafish] Embryo parisites, coleps, "twirlies" Message-ID: <67e02e81-a3a3-4720-bc99-30472cfc4a5f@m34g2000hsf.googlegroups.com> ________________________________________________________________________________ I am looking for more information about coleps. These are a small protazoa which parasatize larval zebrafish. Under a dissecting scope they look like little transparent hyperactive beans, less than one hundredth the size of a zebrafish egg, comparable to a zfish blood cell in size. I am only able to see them at >5X mag. -Here's a little mention in an old Zebrafish Science Monitor: http://zfin.org/zf_info/monitor/vol5.1/vol5.1.html#Coleps,%20Scourge%20of%20the%20Baby%20Zebrafish Three seperate zebrafish labs at which I have worked have had minor problems with these little guys (Perhaps I am the vector, dunno). -Does anyone know more about these things than what is stated in the article linked above? E.g. Life cycle, response to dessication, drugs with which to kill them? -Anyone had problems with them that they have overcome? What strategies worked? So far the only good strategy I know of is vigilant dish cleaning. -We have lately been having problems in dishes which we have bleached, which is perplexing. Do other people see them surviving bleaching? Killed by bleaching? (We follow the Volhard protocol) -Some folks have suggested raising water conductivity in our system. Anyone agree? -Also, does anyone know if UV can kill these or specifically what levels? -Anyone offering up advice or results regarding these pesky things will be my personal hero. Thanks, Gabe