[Zbrafish] ZebraFish Skin Histology
alzaidan.abdullah from gmail.com
(by alzaidan.abdullah from gmail.com)
Mon Jul 21 20:50:33 EST 2008
Im currently studying the effect of contaminants on the skin of
Zebrafish through Histology. The problem Im facing while processing
the tissue is after doing so the skin always detaches from the muscle.
The fixative used is 4% NBF for 24 hrs. And Im only removing the skin
attached with muscle, also tried to cut a TS section (Dorsal to
Ventral through the vertebra) but similar effect. The thickness used
on microtome is 5um.
Any help would be appreciated
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