[Zbrafish] emerald GFP variant

Gilbert Weidinger via zbrafish%40net.bio.net (by gilbert.weidinger from biotec.tu-dresden.de)
Mon Jun 16 11:59:23 EST 2008


hi,

does anybody know how well the Emerald GFP variant performs in 
zebrafish? According to Roger Tsien (eg. review in Nature Methods 2005, 
vol2, 905) it is superior to EGFP because it folds more efficiently at 
37°C. I wonder whether that's true also at 28°, which would make it 
brighter than EGFP. Does anybody have experience with it?

thanks
gilbert

-- 
-----------------------------------------------------------
Gilbert Weidinger, PhD

Group Leader SFB 655
Biotechnology Center & Center for Regenerative Therapies
Technical University of Dresden

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