[Zbrafish] IACUC approval for commercial vendors
(by trevarro from uoneuro.uoregon.edu)
Mon Jun 23 16:00:20 EST 2008
There are many facets to your recent post. Here
are some considerations that occurred to me:
With respect to a pet store source of fish:
- There are several considerations; availability
and expense, fish genetics and fish pathogens.
- Some pet store fish are extremely robust and fecund.
- Pet stores in general do not always get their
fish from the same source. The store may get them
from different wholesalers and each wholesaler
may get them from different producers. Petco,
however, being a large corporation, is probably
getting their ZF from a single source with a long
- The quality and genetics will vary as the sources change.
- Large non-laboratory fish sources are probably
more likely to harbor diseases.
- Passing fish through pet stores in general
(including large corporate fish stores) often
exposes them to the diseases of fish that have
passed through the fish store in the past.
- These fish may also be easier to get through
the larval stages and therefore useful in
initially developing nursery care.
With respect to how your facility is operating:
- It sounds like you have a very small set-up and
are not breeding your next generation of fish.
You just want to purchase them. This reduces the
importance of maintaining a completely disease
free facility. The losses you might have in your
worst case scenario (all fish dead, pathogens in
all tanks) can be relatively easily fixed (bleach
everything, re-establish proper functioning of
your filtration, and buy new fish).
This won't work in a large facility maintaining
many different lines, some of which may be
genetically unique. Even if the establish lines
are available, getting them and quarantining them
into the facility is a big hassle.
- However, this does not remove your
responsibility of taking good care of your fish,
trying to get healthy ones, and avoiding sick
With respect to the IACUC:
- Try using common sense with the IACUC. What has
the history been of the fish you got from that
source? How many other approved fish sources are
out there? What are the requirements for approval
and how realistically relevant are they to your
zebrafish colony? Records such as longevity of
the different groups of fish may be useful in
With respect to the intended use of the fish:
- If you are not using them for genetics then pet
store fish maybe fine, however, if your are doing
molecular biology them you are doing genetics and
it could matter. Also other factors (such as
disease) may effect experimental results.
>Hi all, especially those of you with insight into veterinarians.
>I have an approved protocol to work with fish embryos; my
>"colony" (school ?) of fish consists of two ten gallon tanks with
>filters etc, about 20 fish, and it is in my lab, not the animal
>quarters. In that protocol I listed ZIRC as the source of the
>adults. I have found, however, that my local Petco sells perfectly
>healthy fish for about $1 each, and shopping there has the advantage
>that I can pick out big fat females and good looking males, and I
>don't have to buy in pairs.
>Here's the but - Petco is not an "approved vendor" and the vet on my
>IACUC committee (on which, by the way, I serve as the fish & amphibian
>expert) has put a hold on my purchasing of new fish until I can get
>some kind of health certificate.
>Has anyone figured out a workaround for this problem ? I'm trying to
>get in contact with the store manager, but it's a Petco for pete's
>sake - what is the likelihood that they will have the kind of
>documentation needed for an IACUC committee composed almost entirely
>of mouse people ?
>Does anyone else just use pet store wildtypes ? Am I making a huge
>mistake by doing that ? (I don't do any genetics, so I don't need
>Any wisdom or practical advice will be very welcome.
>Zbrafish mailing list
>Zbrafish from net.bio.net
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