[Zbrafish] Re: buffer solution

françois via zbrafish%40net.bio.net (by busquet.francois from gmail.com)
Wed Mar 5 03:46:35 EST 2008

On Mar 4, 7:57 pm, shaukat <shaukatali... from gmail.com> wrote:
> I am determining ammonia toxicity on Zebrafish eggs and larvea.I am
> looking a non lethal buffer solution to maintain pH of ammonia
> solutions.

Tris-HCl buffer works for me at 0.1M when dissolved in fish medium;

fish medium:
Salts composition	Concentration [mM]
CaCl2 * 2 H2O	2
MgSO4 * 7 H2O	0.5
NaHCO3	                0.7
KCl	                0.07

hope it can help!

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