[Zbrafish] Re: buffer solution
(by shaukatali134 from gmail.com)
Tue Mar 11 11:37:02 EST 2008
On Mar 5, 9:46 am, françois <busquet.franc... from gmail.com> wrote:
> On Mar 4, 7:57 pm, shaukat <shaukatali... from gmail.com> wrote:
> > I am determining ammonia toxicity on Zebrafish eggs and larvea.I am
> > looking a non lethal buffer solution to maintain pH of ammonia
> > solutions.
> Tris-HCl buffer works for me at 0.1M when dissolved in fish medium;
> fish medium:
> Salts composition Concentration [mM]
> CaCl2 * 2 H2O 2
> MgSO4 * 7 H2O 0.5
> NaHCO3 0.7
> KCl 0.07
> hope it can help!
Thank you very much for your help.
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