From fritsa01 from gettysburg.edu Mon Nov 3 22:40:25 2008 From: fritsa01 from gettysburg.edu (FritzXC23) Date: Tue Nov 4 12:13:00 2008 Subject: [Zbrafish] Albino Zebrafish Message-ID: <568d4ada-66d3-4241-ae87-0ba50af9d99c@w39g2000prb.googlegroups.com> Hi everyone! I am a junior undergraduate biochemistry major at Gettysburg College and I am working on a research project that investigates the effects of vitamin A deficiency on the development of ocular colobomas. I am trying to depigment the zebrafish embryos using phenylthiourea. I was wondering if anyone has used this tyrosinase inhibitor before, and if so at what time and for how long were the embryos dosed in a 0.003% solution of PTU? Thanks so much for your help! From sunyonghua from gmail.com Tue Nov 4 04:49:57 2008 From: sunyonghua from gmail.com (Clonfish) Date: Tue Nov 4 12:13:24 2008 Subject: [Zbrafish] about phenol red solution for microinjection Message-ID: <89f0e26f-4a1d-4739-84de-35cbcd9c43e5@t18g2000prt.googlegroups.com> Dear all, I am trying to make a 5X stock phenol red solution for microinjection. When I searched the literature, I found different formulas from different papers, e.g., (0.1% phenol red, 100mM KCl) or (0.5% phenol red, 240 mM KCl) or (1% phenol red, 200mM KCl). Do you have any idea that which one gives the best result in your hands? Another question is that when I prepared 1.2% phenol red solution, it was really hard for the phenol red to be dissolved completely. Does anybody have any suggestion for that? Thanks a lot! Yonghua From rburdine from Princeton.EDU Tue Nov 4 13:48:29 2008 From: rburdine from Princeton.EDU (Burdine, Rebecca D) Date: Tue Nov 4 13:53:17 2008 Subject: [Zbrafish] Albino Zebrafish In-Reply-To: <568d4ada-66d3-4241-ae87-0ba50af9d99c@w39g2000prb.googlegroups.com> References: <568d4ada-66d3-4241-ae87-0ba50af9d99c@w39g2000prb.googlegroups.com> Message-ID: <04AF14C4EF3CF34682460FB1FDBA98C03F9701@MBCLUSTER.pu.win.princeton.edu> Hi, If you want to inhibit most of the melanin, we put embryos into the PTU solution during somitogenesis and then raise them in the solution from this point on. If you take them out of the solution, melanin synthesis can resume. Two key things we have found to be important when suing PTU: 1) We make this fresh every two weeks or so and keep it in a bottle wrapped in foil. 2) You need to change the embryo media containing PTU everyday to keep the melanin production down and the embryos happy and healthy. (Of course you have to remove all the dying embryos and debris too as you would normally do.) Hope this helps, Becky --------------------------------------------------- Rebecca D. Burdine, Ph.D. Assistant Professor Dept. of Molecular Biology Princeton University Washington Road Mof 433 Princeton, NJ 08544 Phone: (609) 258-7515 Fax: (609) 258-1343 Email: rburdine@princeton.edu Admin Assistant: Cathy Falk (609) 258-1604 > -----Original Message----- > From: zbrafish-bounces@oat.bio.indiana.edu [mailto:zbrafish- > bounces@oat.bio.indiana.edu] On Behalf Of FritzXC23 > Sent: Monday, November 03, 2008 10:40 PM > To: bionet-organisms-zebrafish@moderators.isc.org > Subject: [Zbrafish] Albino Zebrafish > > Hi everyone! I am a junior undergraduate biochemistry major at > Gettysburg College and I am working on a research project that > investigates the effects of vitamin A deficiency on the development of > ocular colobomas. I am trying to depigment the zebrafish embryos using > phenylthiourea. I was wondering if anyone has used this tyrosinase > inhibitor before, and if so at what time and for how long were the > embryos dosed in a 0.003% solution of PTU? Thanks so much for your > help! > > _______________________________________________ > Zbrafish mailing list > Zbrafish@net.bio.net > http://www.bio.net/biomail/listinfo/zbrafish From zhangch from ohsu.edu Tue Nov 4 15:16:15 2008 From: zhangch from ohsu.edu (Chao Zhang) Date: Tue Nov 4 17:10:30 2008 Subject: [Zbrafish] TU wild type fish Message-ID: Is anybody using TU wild type fish? We need a little bit (10 embryos) for our research. Thanks. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/zbrafish/attachments/20081104/c79ce339/attachment.html From david from zebrafish.org Tue Nov 4 17:35:56 2008 From: david from zebrafish.org (David Lains) Date: Tue Nov 4 17:46:53 2008 Subject: [Zbrafish] TU wild type fish In-Reply-To: References: Message-ID: <06df01c93ecd$b718e690$254ab3b0$@org> Hello Chao Zhang The Tu line is available as embryos from ZIRC. If you would like you can place an order at www.zebrafish.org. Please feel free to contact me if you have any questions or concerns. Best Fishes David Lains <}}}>< Aquaculturist, Research Assistant Zebrafish International Resource Center 5274 University of Oregon Eugene, Or 97403 Email: david@zebrafish.org pH: (541) 346-6028 ext. 18 fax: (541) 346-6151 From: zbrafish-bounces@oat.bio.indiana.edu [mailto:zbrafish-bounces@oat.bio.indiana.edu] On Behalf Of Chao Zhang Sent: Tuesday, November 04, 2008 12:16 PM To: zbrafish@magpie.bio.indiana.edu Subject: [Zbrafish] TU wild type fish Is anybody using TU wild type fish? We need a little bit (10 embryos) for our research. Thanks. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/zbrafish/attachments/20081104/6a5f8a6a/attachment.html From caroline.parkin from sheffield.ac.uk Wed Nov 5 12:17:32 2008 From: caroline.parkin from sheffield.ac.uk (Caroline Parkin) Date: Wed Nov 5 12:29:58 2008 Subject: [Zbrafish] Re: Phenol Red In-Reply-To: <200811051703.mA5H39V05687@net.bio.net> References: <200811051703.mA5H39V05687@net.bio.net> Message-ID: Regarding phenol red, we use it direct (unmodified) from Sigma,catalog number P0290, at around 5-10% concentration in our injection mix. Hope that helps, Caroline On 5 Nov 2008, at 17:03, zbrafish-request@oat.bio.indiana.edu wrote: > Send Zbrafish mailing list submissions to > zbrafish@net.bio.net > > To subscribe or unsubscribe via the World Wide Web, visit > http://www.bio.net/biomail/listinfo/zbrafish > or, via email, send a message with subject or body 'help' to > zbrafish-request@net.bio.net > > You can reach the person managing the list at > zbrafish-owner@net.bio.net > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Zbrafish digest..." > > > Today's Topics: > > 1. Albino Zebrafish (FritzXC23) > 2. about phenol red solution for microinjection (Clonfish) > 3. RE: Albino Zebrafish (Burdine, Rebecca D) > 4. TU wild type fish (Chao Zhang) > 5. RE: TU wild type fish (David Lains) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 3 Nov 2008 19:40:25 -0800 (PST) > From: FritzXC23 > Subject: [Zbrafish] Albino Zebrafish > To: bionet-organisms-zebrafish@moderators.isc.org > Message-ID: > <568d4ada-66d3-4241-ae87-0ba50af9d99c@w39g2000prb.googlegroups.com> > Content-Type: text/plain; charset=ISO-8859-1 > > Hi everyone! I am a junior undergraduate biochemistry major at > Gettysburg College and I am working on a research project that > investigates the effects of vitamin A deficiency on the development of > ocular colobomas. I am trying to depigment the zebrafish embryos using > phenylthiourea. I was wondering if anyone has used this tyrosinase > inhibitor before, and if so at what time and for how long were the > embryos dosed in a 0.003% solution of PTU? Thanks so much for your > help! > > > > ------------------------------ > > Message: 2 > Date: Tue, 4 Nov 2008 01:49:57 -0800 (PST) > From: Clonfish > Subject: [Zbrafish] about phenol red solution for microinjection > To: bionet-organisms-zebrafish@moderators.isc.org > Message-ID: > <89f0e26f-4a1d-4739-84de-35cbcd9c43e5@t18g2000prt.googlegroups.com> > Content-Type: text/plain; charset=ISO-8859-1 > > Dear all, > > I am trying to make a 5X stock phenol red solution for microinjection. > When I searched the literature, I found different formulas from > different papers, e.g., (0.1% phenol red, 100mM KCl) or (0.5% phenol > red, 240 mM KCl) or (1% phenol red, 200mM KCl). Do you have any idea > that which one gives the best result in your hands? > > Another question is that when I prepared 1.2% phenol red solution, it > was really hard for the phenol red to be dissolved completely. Does > anybody have any suggestion for that? > > Thanks a lot! > > Yonghua > > > > ------------------------------ > > Message: 3 > Date: Tue, 4 Nov 2008 13:48:29 -0500 > From: "Burdine, Rebecca D" > Subject: RE: [Zbrafish] Albino Zebrafish > To: "FritzXC23" > Cc: Zbrafish@magpie.bio.indiana.edu > Message-ID: > <04AF14C4EF3CF34682460FB1FDBA98C03F9701@MBCLUSTER.pu.win.princeton.edu > > > > Content-Type: text/plain; charset="us-ascii" > > Hi, > > If you want to inhibit most of the melanin, we put embryos into the > PTU > solution during somitogenesis and then raise them in the solution from > this point on. If you take them out of the solution, melanin > synthesis > can resume. > > Two key things we have found to be important when suing PTU: 1) We > make > this fresh every two weeks or so and keep it in a bottle wrapped in > foil. 2) You need to change the embryo media containing PTU everyday > to > keep the melanin production down and the embryos happy and healthy. > (Of > course you have to remove all the dying embryos and debris too as you > would normally do.) > > Hope this helps, > Becky > > --------------------------------------------------- > Rebecca D. Burdine, Ph.D. > Assistant Professor > Dept. of Molecular Biology > Princeton University > Washington Road Mof 433 > Princeton, NJ 08544 > > Phone: (609) 258-7515 > Fax: (609) 258-1343 > Email: rburdine@princeton.edu > Admin Assistant: Cathy Falk (609) 258-1604 > >> -----Original Message----- >> From: zbrafish-bounces@oat.bio.indiana.edu [mailto:zbrafish- >> bounces@oat.bio.indiana.edu] On Behalf Of FritzXC23 >> Sent: Monday, November 03, 2008 10:40 PM >> To: bionet-organisms-zebrafish@moderators.isc.org >> Subject: [Zbrafish] Albino Zebrafish >> >> Hi everyone! I am a junior undergraduate biochemistry major at >> Gettysburg College and I am working on a research project that >> investigates the effects of vitamin A deficiency on the development >> of >> ocular colobomas. I am trying to depigment the zebrafish embryos >> using >> phenylthiourea. I was wondering if anyone has used this tyrosinase >> inhibitor before, and if so at what time and for how long were the >> embryos dosed in a 0.003% solution of PTU? Thanks so much for your >> help! >> >> _______________________________________________ >> Zbrafish mailing list >> Zbrafish@net.bio.net >> http://www.bio.net/biomail/listinfo/zbrafish > > > > ------------------------------ > > Message: 4 > Date: Tue, 4 Nov 2008 12:16:15 -0800 > From: "Chao Zhang" > Subject: [Zbrafish] TU wild type fish > To: zbrafish@magpie.bio.indiana.edu > Message-ID: > Content-Type: text/plain; charset="gb2312" > > Is anybody using TU wild type fish? > We need a little bit (10 embryos) for our research. > Thanks. > -------------- next part -------------- > An HTML attachment was scrubbed... > URL: http://www.bio.net/bionet/mm/zbrafish/attachments/20081104/c79ce339/attachment-0001.html > > ------------------------------ > > Message: 5 > Date: Tue, 4 Nov 2008 14:35:56 -0800 > From: "David Lains" > Subject: RE: [Zbrafish] TU wild type fish > To: "'Chao Zhang'" , > > Message-ID: <06df01c93ecd$b718e690$254ab3b0$@org> > Content-Type: text/plain; charset="us-ascii" > > Hello Chao Zhang > > > > The Tu line is available as embryos from ZIRC. > > > > If you would like you can place an order at www.zebrafish.org. > > > > Please feel free to contact me if you have any questions or concerns. > > > > Best Fishes > David Lains <}}}>< > Aquaculturist, Research Assistant > Zebrafish International Resource Center > 5274 University of Oregon > Eugene, Or 97403 > > Email: david@zebrafish.org > pH: (541) 346-6028 ext. 18 > fax: (541) 346-6151 > > > > From: zbrafish-bounces@oat.bio.indiana.edu > [mailto:zbrafish-bounces@oat.bio.indiana.edu] On Behalf Of Chao Zhang > Sent: Tuesday, November 04, 2008 12:16 PM > To: zbrafish@magpie.bio.indiana.edu > Subject: [Zbrafish] TU wild type fish > > > > Is anybody using TU wild type fish? > We need a little bit (10 embryos) for our research. > Thanks. > > -------------- next part -------------- > An HTML attachment was scrubbed... > URL: http://www.bio.net/bionet/mm/zbrafish/attachments/20081104/6a5f8a6a/attachment-0001.html > > ------------------------------ > > _______________________________________________ > Zbrafish mailing list > Zbrafish@net.bio.net > http://www.bio.net/biomail/listinfo/zbrafish > > End of Zbrafish Digest, Vol 42, Issue 1 > *************************************** From wclements from ucsd.edu Wed Nov 5 12:51:42 2008 From: wclements from ucsd.edu (Wilson Clements) Date: Wed Nov 5 13:37:30 2008 Subject: [Zbrafish] about phenol red solution for microinjection Message-ID: <9F2CA893-1189-4722-88FF-B4D075A385ED@ucsd.edu> Dear Yonghua, We use 0.5% phenol red in DPBS (Sigma, cat. no. P-0290) This solution comes already made. I use it at 5X (1ul per 5ul total dilution). Best, Wilson Message: 2 Date: Tue, 4 Nov 2008 01:49:57 -0800 (PST) From: Clonfish Subject: [Zbrafish] about phenol red solution for microinjection To: bionet-organisms-zebrafish@moderators.isc.org Message-ID: <89f0e26f-4a1d-4739-84de-35cbcd9c43e5@t18g2000prt.googlegroups.com> Content-Type: text/plain; charset=ISO-8859-1 Dear all, I am trying to make a 5X stock phenol red solution for microinjection. When I searched the literature, I found different formulas from different papers, e.g., (0.1% phenol red, 100mM KCl) or (0.5% phenol red, 240 mM KCl) or (1% phenol red, 200mM KCl). Do you have any idea that which one gives the best result in your hands? Another question is that when I prepared 1.2% phenol red solution, it was really hard for the phenol red to be dissolved completely. Does anybody have any suggestion for that? Thanks a lot! Yonghua ------------------------------------------------------------------------ -------------------- Wilson Clements, Ph.D. wclements@ucsd.edu Dept. of Biology Section of Cell and Developmental Biology University of California at San Diego 9500 Gilman Dr. Natural Sciences Building 6105 La Jolla, CA 92093-0380 TEL (858) 534-6955 LAB (858) 822-4658 FAX (858) 822-5740 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/zbrafish/attachments/20081105/4318e490/attachment.html From amp from stowers-institute.org Thu Nov 6 23:47:28 2008 From: amp from stowers-institute.org (amp@stowers-institute.org) Date: Fri Nov 7 11:53:43 2008 Subject: [Zbrafish] Feeding of Artemia nauplii Message-ID: <6d7e2dfc-430b-4baa-8cbc-ddb0a8508134@s1g2000prg.googlegroups.com> For feeding Artemia nauplii to Zebrafish, are there any guidelines on a specific amount to feed (i.e. dry weight or nauplii per fish) or is it based more on observation (i.e. the fish eat about this much in X minutes)? Thanks, Adam Petrie Laboratory Supervisor Aquatics Facility Stowers Institute for Medical Research Kansas City, MO From ab from anaspec.com Tue Nov 11 18:02:11 2008 From: ab from anaspec.com (Antibody) Date: Tue Nov 11 18:07:54 2008 Subject: [Zbrafish] Antibody for SOX9a Zebrafish Message-ID: <00b401c94451$8827cfc0$0201a8c0@anaspec03> Hi Jorge, AnaSpec sells rabbit polyclonal anti-SOX9 antibody (cat# 28138) which will work for zebrafish SOX9a. Warm regards, Daniel [Zbrafish] Antibody for SOX9a Zebrafish Jorge Soares via zbrafish%40net.bio.net (by j.s.soares from gmail.com) Wed Aug 1 08:07:04 EST 2007 a.. Next message: [Zbrafish] PH meter b.. Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] -------------------------------------------------------------------------------- Hi everyone, Just recently joined this mailing list, mainly because I'm searching for an Antibody (mono or poli) that binds to SOX9a from Zebrafish, still leaving the TFBS (transcription Factor Bionding Site) open for it to be able to link to the DNA i'm studying. Does anyone know of a company that produces it? Cheers. Jorge -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/zbrafish/attachments/20070801/9cfd411f/attachment.html -- I am using the free version of SPAMfighter. We are a community of 5.6 million users fighting spam. SPAMfighter has removed 63706 of my spam emails to date. Get the free SPAMfighter here: http://www.spamfighter.com/len The Professional version does not have this message -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/zbrafish/attachments/20081111/78a0c52e/attachment.html From mena22787 from excite.com Fri Nov 14 15:08:02 2008 From: mena22787 from excite.com (Serena) Date: Fri Nov 14 15:10:23 2008 Subject: [Zbrafish] Re: Feeding of Artemia nauplii References: Message-ID: <6e524d03-136d-4ba8-b942-78edd4a657f5@n33g2000pri.googlegroups.com> On Nov 6, 11:47 pm, a...@stowers-institute.org wrote: > For feeding Artemia nauplii to Zebrafish, are there any guidelines on > a specific amount to feed (i.e. dry weight or nauplii per fish) or is > it based more on observation (i.e. the fish eat about this much in X > minutes)? > > Thanks, > > Adam Petrie > Laboratory Supervisor > Aquatics Facility > Stowers Institute for Medical Research > Kansas City, MO we just feed them a couple pipette-fulls of the artemia, supplemented with dry pellets. From pmarvit from gmail.com Mon Nov 17 12:52:21 2008 From: pmarvit from gmail.com (Peter Marvit) Date: Mon Nov 17 14:23:53 2008 Subject: [Zbrafish] Feeding time(s) and light cycles for zf? Message-ID: <3f6d24a40811170952p21f32b30r1a0b5d5dfbb0a0e0@mail.gmail.com> OK, a naive question: What are the light cycles that folks use for their zebrafish colonies? Do you differentiate between adults who are breeding versus young or adults who will not be breeding? As a corollary, when do you feed your colonies? Are there any noticeable differences, for example, between feeding in the morning or afternoon or soon before "lights out"? Do you do just one or more than one daily feeding? Or do you have an "every nth-day" schedule? I'm anticipating restarting a very small experimental school of fish, but not for breeding purposes. And, yes, I need to go back through the archives for food suppliers.... Cheers, Peter -- : Peter Marvit, PhD : : Psychology Department, University of Maryland, College Park : College Park, MD 20742 : (lab) 301-405-5940 (fax) 301-314-9566 From mwinandy from ulg.ac.be Wed Nov 19 03:22:22 2008 From: mwinandy from ulg.ac.be (Marie Winandy) Date: Wed Nov 19 11:52:13 2008 Subject: [Zbrafish] Re: Feeding time(s) and light cycles for zf In-Reply-To: <200811181703.mAIH3FV13973@net.bio.net> References: <200811181703.mAIH3FV13973@net.bio.net> Message-ID: <200811190922220515.00157C5D@smtp.ulg.ac.be> Hi Peter and all, We use a 14h light / 10h dark cycle, both for adults and fry (they are in the same room). The light turns on at 8.30 am and off at 10.30 pm. We feed the fish 3 times a day (on working days) : dry food between 9 and 10 am, artemias at 2 pm and dry food again at 4 pm. The very young have also paramecias once a day (in the morning). In the week-end fishreceive artemias and dry food with an interval of 1 hour (dry food only once). If you don't need the fish to breed, I think you can give them only dry food, once a day, it will be enough to maintain them. Artemias and more dry food are required if you need a lot of eggs. Best regards, M. Winandy Université de Liège GIGA B34 - Zebrafish Platform Avenue de l'Hôpital 1 B-4000 Liège - Sart Tilman Belgique Tél: +32.4.366.99.71 +32.4.366.33.38 +32.476.97.25.33 Fax: +32.4.366.41.98 email : mwinandy@ulg.ac.be or zebrafish.giga@ulg.ac.be http://www.giga.ulg.ac.be/extranet/services.htm From jason.cockington from gmail.com Thu Nov 20 22:13:05 2008 From: jason.cockington from gmail.com (Leviathan) Date: Fri Nov 21 12:22:58 2008 Subject: [Zbrafish] Re: Feeding time(s) and light cycles for zf? References: Message-ID: Hi peter et al., We also use a 14/10 cycle. Our rooms come on at 8am, and our brood tanks have dawns at 9am, 11am, 1pm, and 2pm. Plenty of egg collecting time! We also feed multiple times throughout the day, day light fish get fed at 9, 11, and 2pm with INVE NRD5/8 and Skretting PerlaAQ. Then all fish receive an artemia feed at 4pm ish. Our fry get fed golden pearls throughout the day, as well as receiving green water and artemia. http://www.nezhaonline.org/library_items/7/Danio_rerio_Larval_Rearing_Protocol.doc hope this helps, jason From raschperger.work from hotmail.com Fri Nov 21 09:41:58 2008 From: raschperger.work from hotmail.com (raschperger.work@hotmail.com) Date: Fri Nov 21 12:23:25 2008 Subject: [Zbrafish] ZF survival without heartbeat Message-ID: Dear all, During early development (1-7 dpf), does zf embryos/larvae survive without heartbeating. Do they develop normal the first coulple of days if they dont have a functional heart? How is the development of the vasculature affected if the heart doesnt beat? Thank you! From Thomas.Bartman from cchmc.org Fri Nov 21 13:27:28 2008 From: Thomas.Bartman from cchmc.org (Thomas Bartman) Date: Fri Nov 21 13:33:45 2008 Subject: [Zbrafish] ZF survival without heartbeat Message-ID: <4926B7400200007600021A4C@n6mcgw16.cchmc.org> In general, the embryos seem to develop normally from 22 hpf (when heartbeat would initiate) until 3-4 dpf. They will develop edema around the heart, and for some reason the eyes usually seem to be smaller in heart mutants, but otherwise they seem fine. The question about vascular development is a good one, for which I have no specific answer. By flk1::GFP, endothelial cells seem to be there, and normally aligned, but I haven't checked to see if there is an actual lumen to the vessels, or if it is collapsed, or atretic (different things). After 4-5 dpf or so, the embryos gradually succumb to hypoxia and death. Thomas Bartman, M.D., Ph.D. Cincinnati Children's Hospital Divisions of Neonatology, Pulmonary Biology and Developmental Biology 3333 Burnet Avenue Cincinnati, OH 45229 (513) 636-9902 >>> 11/21/08 9:41 AM >>> Dear all, During early development (1-7 dpf), does zf embryos/larvae survive without heartbeating. Do they develop normal the first coulple of days if they dont have a functional heart? How is the development of the vasculature affected if the heart doesnt beat? Thank you! _______________________________________________ Zbrafish mailing list Zbrafish@net.bio.net http://www.bio.net/biomail/listinfo/zbrafish From pmarvit from gmail.com Fri Nov 21 17:28:09 2008 From: pmarvit from gmail.com (Peter Marvit) Date: Fri Nov 21 17:42:14 2008 Subject: [Zbrafish] Re: Feeding time(s) and light cycles for zf In-Reply-To: <200811190922220515.00157C5D@smtp.ulg.ac.be> References: <200811181703.mAIH3FV13973@net.bio.net> <200811190922220515.00157C5D@smtp.ulg.ac.be> Message-ID: <3f6d24a40811211428j3ad2f93dk9962211a087bddb3@mail.gmail.com> A public thanks to all who responded to my plea for information. As always, members of the Zebrafish list are unfailing in their generosity and helpfulness. With luck, in some months, I should be able to report some success with our experiments. Cheers, Peter -- : Peter Marvit, PhD : : Psychology Department, University of Maryland, College Park : College Park, MD 20742 : (lab) 301-405-5940 (fax) 301-314-9566 From fritsa01 from gettysburg.edu Sat Nov 22 08:15:10 2008 From: fritsa01 from gettysburg.edu (FritzXC23) Date: Sat Nov 22 16:44:44 2008 Subject: [Zbrafish] Zebrafish Disease Message-ID: Hi Everyone, I have noticed recently that many of my fish are dying. They all have a characterisitc "burn-mark" (almost like a bright red skin abrasion) on their sides. I was wondering if anyone has seen this in their fish before and know if it is a disease and how to treat them. Thanks! Sarah From zoltan from zebrafish.org Fri Nov 21 18:20:18 2008 From: zoltan from zebrafish.org (Zoltan) Date: Sat Nov 22 16:45:09 2008 Subject: [Zbrafish] Help! Do you have access to this publication! Message-ID: <0d0fd348-69aa-482b-9fc8-d1ee5e38359a@t39g2000prh.googlegroups.com> Hi, The ZIRC team needs a PDF or a good photocopy of this article, but it is not available through Pubmed/ZFIN. If you could locate this Journal and send us a (PDF) copy - it would help us tremendously! Ewing, H.H. (1972) Spermatogenesis in the zebrafish, Brachydanio rerio. Anat. Rec. 172: 308. Thank you very much! Zoltan From gilbert.weidinger from biotec.tu-dresden.de Mon Nov 24 12:38:50 2008 From: gilbert.weidinger from biotec.tu-dresden.de (Gilbert Weidinger) Date: Mon Nov 24 13:07:55 2008 Subject: [Zbrafish] Postdoc position Message-ID: <492AE6AA.9090608@biotec.tu-dresden.de> A postdoctoral position is available in the Weidinger group at the Biotechnology Center of the Technische Universit?t Dresden, Germany. Our group studies the signaling pathways regulating regeneration of the zebrafish fin and heart. See http://www.biotec.tu-dresden.de/weidinger for further information. We are looking for a motivated, creative researcher with a track record of peer-reviewed publications. Candidates should have a strong background in Developmental or Cell Biology, be skilled in molecular biology methods and must have received, or are soon expecting, a PhD degree. Experience with the zebrafish model and imaging techniques would be advantageous, but is not required. We offer a stimulating working environment in a young, motivated team. The life-sciences campus in Dresden provides a very competitive research setting that includes the Max-Planck Institute for Cell Biology and Genetics, the Center for Regenerative Therapies Dresden, the Medical Theoretical Center (MTZ) of the Medical School, and our institute, the Biotechnology Center (Biotec). The Biotec is an interdisciplinary research institute that houses cutting edge facilities including Imaging, Proteomics, Histology, and a large fish facility. There are currently 5 groups working with zebrafish on campus, forming a lively fish community. Last not least, Dresden is a vibrant city that offers many cultural opportunities, cheap cost of living and easy access to great outdoor activities. Applications received by March 31^st , 2009 will get full consideration. We will continue to review applications until the position is filled. Please send a CV, a list of publications, a summary of past/current research (1-2 pages) and the contact information of 2 or 3 referees to Gilbert Weidinger gilbert.weidinger@biotec.tu-dresden.de . Women are specifically encouraged to apply. Handicapped applicants are favored given otherwise identical qualification. -- -------------------------------------------------------- Gilbert Weidinger, PhD Group Leader SFB 655 Biotechnology Center & Center for Regenerative Therapies University of Technology Dresden Mail address: Gilbert Weidinger Biotechnology Center Tatzberg 47-51 01307 Dresden Germany Tel. office: 0049 351 463 40120 Tel. lab: 0049 351 463 40108 Tel. secretary: 0049 351 463 40345 Fax: 0049 351 463 40348 email: gilbert.weidinger@biotec.tu-dresden.de web: http://www.biotec.tu-dresden.de/weidinger From cayuelamarialuisa from gmail.com Wed Nov 26 07:20:21 2008 From: cayuelamarialuisa from gmail.com (MariaLuisa Cayuela) Date: Wed Nov 26 12:35:04 2008 Subject: [Zbrafish] zebrafish company Message-ID: <84e45b690811260420q181a9206xe1be51e363ebb6ed@mail.gmail.com> Hello Everyone, I would like to find a company wich made transgenic zebrafish with a modiefied BAC Samebody know one company that do it??? Thanks!!! Have a nice day Mar?a -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/zbrafish/attachments/20081126/d5d5f685/attachment.html From sunyonghua from gmail.com Wed Nov 26 08:54:26 2008 From: sunyonghua from gmail.com (Clonfish) Date: Wed Nov 26 12:35:38 2008 Subject: [Zbrafish] Re: Help! Do you have access to this publication! References: Message-ID: <7f09ac38-5359-441a-8d45-a36f7fef39ae@a12g2000pro.googlegroups.com> On Nov 22, 7:20?am, Zoltan wrote: > Hi, > > The ZIRC team needs a PDF or a good photocopy of this article, but it > is not available through Pubmed/ZFIN. If you could locate this Journal > and send us a (PDF) copy - it would help us tremendously! > > Ewing, H.H. (1972) Spermatogenesis in the zebrafish, Brachydanio > rerio. Anat. Rec. 172: 308. > > Thank you very much! > > Zoltan Hi Zoltan, a pdf file of this article is attached. In fact, it is just an abstract. Best, Yonghua From thomas.bartman from cchmc.org Wed Nov 26 10:56:33 2008 From: thomas.bartman from cchmc.org (Thomas Bartman) Date: Wed Nov 26 12:35:59 2008 Subject: [Zbrafish] detecting 2 bp SSLP Message-ID: <20081126160512.0CB2717CB@mx8.cchmc.org> We're doing a positional cloning project and have an SSLP marker with a 2-bp polymorphism. We can pick up the polymorphism with SSCP non-denaturing, cold acrylamide gels sometimes, but not reliably on even high percentage agarose gels. Has anyone used denaturing acrylamide gels for this purpose, and do you have a protocol for the gel composition and sample prep before loading? Would it be the same as what is used for sequencing gels? Thomas Bartman, M.D., Ph.D. Divisions of Neonatology, Pulmonary Biology, and Developmental Biology Cincinnati Children's Hospital Medical Center 3333 Burnet Ave, MLC 7009 Cincinnati, OH 45229-3039 Office: 513-636-9902 From chi-bin.chien from neuro.utah.edu Thu Nov 27 15:07:53 2008 From: chi-bin.chien from neuro.utah.edu (Chi-Bin Chien) Date: Mon Dec 1 12:16:57 2008 Subject: [Zbrafish] detecting 2 bp SSLP In-Reply-To: <20081126160512.0CB2717CB@mx8.cchmc.org> References: <20081126160512.0CB2717CB@mx8.cchmc.org> Message-ID: Tom--if this is a marker you'll use a lot, it may be easier to amplify and sequence a few hundred bp of neighboring genomic DNA from the two mapping strains, find a good SNP, and develop a CAPS or dCAPS marker. Chi-Bin Chien At 10:56 AM -0500 11/26/08, Thomas Bartman wrote: >We're doing a positional cloning project and have an SSLP marker >with a 2-bp polymorphism. We can pick up the polymorphism with SSCP >non-denaturing, cold acrylamide gels sometimes, but not reliably on >even high percentage agarose gels. Has anyone used denaturing >acrylamide gels for this purpose, and do you have a protocol for the >gel composition and sample prep before loading? Would it be the >same as what is used for sequencing gels? > > > >Thomas Bartman, M.D., Ph.D. >Divisions of Neonatology, Pulmonary Biology, and Developmental Biology >Cincinnati Children's Hospital Medical Center >3333 Burnet Ave, MLC 7009 >Cincinnati, OH 45229-3039 > >Office: 513-636-9902 >_______________________________________________ >Zbrafish mailing list >Zbrafish@net.bio.net >http://www.bio.net/biomail/listinfo/zbrafish