[Zbrafish] embryo nuclear extract

Daphne Georlette via zbrafish%40net.bio.net (by daph from temple.edu)
Thu Sep 18 16:31:40 EST 2008

Hi everyone
I'm new in the zebrafish field and I'm trying to find a protocol to  
make nuclear extracts from zebrafish embryos. I was thinking of using  
the low salt/high salt extraction method, but was wondering if any of  
you had a protocol working nicely!

Daphne Georlette, Ph.D.
Research Assistant Professor
Biemar & Georlette Lab
Department of Biology
Temple University
436 Biology Life Sciences Building
1900 North 12th Street
Philadelphia, PA 19122, USA
Tel. 1-215-204-0291
Fax. 1-215-204-6646


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